Pre-existing immunity does not impair the engraftment of CRISPR-Cas9-edited cells in rhesus macaques conditioned with busulfan or radiation

Pre-existing immunity does not impair the engraftment of CRISPR-Cas9-edited cells in rhesus macaques conditioned with busulfan or radiation

CRISPR-Cas9-based therapeutic genome editing approaches hold promise to cure a variety of human diseases. Recent findings demonstrate pre-existing immunity for the commonly used Cas orthologs from Streptococcus pyogenes (SpCas9) and Staphylococcus aureus (SaCas9) in humans, which threatens the succe...

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Bibliographic Details
Main Authors: Khaled Essawi, Waleed Hakami, Muhammad Behroz Naeem Khan, Reid Martin, Jing Zeng, Rebecca Chu, Naoya Uchida, Aylin C. Bonifacino, Allen E. Krouse, Nathaniel S. Linde, Robert E. Donahue, Gerd A. Blobel, Ulrike Gerdemann, Leslie S. Kean, Stacy A. Maitland, Scot A. Wolfe, Jean-Yves Metais, Stephen Gottschalk, Daniel E. Bauer, John F. Tisdale, Selami Demirci
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
SaCas9
SpCas9
genome editing
gene therapy
immunity
hematopoietic stem cell transplantation
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050123000578

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http://www.sciencedirect.com/science/article/pii/S2329050123000578

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