Continuous Production of Human Epidermal Growth Factor Using Escherichia coli Biofilm
Increasing demand for recombinant proteins necessitates efficient protein production processes. In this study, a continuous process for human epidermal growth factor (hEGF) secretion by Escherichia coli was developed by taking advantage of biofilm formation. Genes bcsB, fimH, and csgAcsgB that have...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.855059/full |
| _version_ | 1819085263356297216 |
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| author | Mengting Li Mengting Li Zhenyu Wang Miao Zhou Chong Zhang Kaiqi Zhi Shuli Liu Xiujuan Sun Zhi Wang Jinle Liu Dong Liu Dong Liu Dong Liu |
| author_facet | Mengting Li Mengting Li Zhenyu Wang Miao Zhou Chong Zhang Kaiqi Zhi Shuli Liu Xiujuan Sun Zhi Wang Jinle Liu Dong Liu Dong Liu Dong Liu |
| author_sort | Mengting Li |
| collection | DOAJ |
| description | Increasing demand for recombinant proteins necessitates efficient protein production processes. In this study, a continuous process for human epidermal growth factor (hEGF) secretion by Escherichia coli was developed by taking advantage of biofilm formation. Genes bcsB, fimH, and csgAcsgB that have proved to facilitate biofilm formation and some genes moaE, yceA, ychJ, and gshB potentially involved in biofilm formation were examined for their effects on hEGF secretion as well as biofilm formation. Finally, biofilm-based fermentation processes were established, which demonstrated the feasibility of continuous production of hEGF with improved efficiency. The best result was obtained from ychJ-disruption that showed a 28% increase in hEGF secretion over the BL21(DE3) wild strain, from 24 to 32 mg/L. Overexpression of bcsB also showed great potential in continuous immobilized fermentation. Overall, the biofilm engineering here represents an effective strategy to improve hEGF production and can be adapted to produce more recombinant proteins in future. |
| first_indexed | 2024-12-21T21:01:34Z |
| format | Article |
| id | doaj.art-1890b6cd8fde4890a7b37eff581b21cb |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-21T21:01:34Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1890b6cd8fde4890a7b37eff581b21cb2022-12-21T18:50:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-04-011310.3389/fmicb.2022.855059855059Continuous Production of Human Epidermal Growth Factor Using Escherichia coli BiofilmMengting Li0Mengting Li1Zhenyu Wang2Miao Zhou3Chong Zhang4Kaiqi Zhi5Shuli Liu6Xiujuan Sun7Zhi Wang8Jinle Liu9Dong Liu10Dong Liu11Dong Liu12State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, ChinaSchool of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou, ChinaSchool of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou, ChinaInstitute of Industrial Biotechnology, Jiangsu Industrial Technology Research Institute (JITRI), Nanjing, ChinaSchool of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou, ChinaSchool of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, ChinaSchool of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou, ChinaInstitute of Industrial Biotechnology, Jiangsu Industrial Technology Research Institute (JITRI), Nanjing, ChinaIncreasing demand for recombinant proteins necessitates efficient protein production processes. In this study, a continuous process for human epidermal growth factor (hEGF) secretion by Escherichia coli was developed by taking advantage of biofilm formation. Genes bcsB, fimH, and csgAcsgB that have proved to facilitate biofilm formation and some genes moaE, yceA, ychJ, and gshB potentially involved in biofilm formation were examined for their effects on hEGF secretion as well as biofilm formation. Finally, biofilm-based fermentation processes were established, which demonstrated the feasibility of continuous production of hEGF with improved efficiency. The best result was obtained from ychJ-disruption that showed a 28% increase in hEGF secretion over the BL21(DE3) wild strain, from 24 to 32 mg/L. Overexpression of bcsB also showed great potential in continuous immobilized fermentation. Overall, the biofilm engineering here represents an effective strategy to improve hEGF production and can be adapted to produce more recombinant proteins in future.https://www.frontiersin.org/articles/10.3389/fmicb.2022.855059/fullEscherichia colibiofilm formationhEGF secretionimmobilized fermentationbiofilm-based fermentation |
| spellingShingle | Mengting Li Mengting Li Zhenyu Wang Miao Zhou Chong Zhang Kaiqi Zhi Shuli Liu Xiujuan Sun Zhi Wang Jinle Liu Dong Liu Dong Liu Dong Liu Continuous Production of Human Epidermal Growth Factor Using Escherichia coli Biofilm Frontiers in Microbiology Escherichia coli biofilm formation hEGF secretion immobilized fermentation biofilm-based fermentation |
| title | Continuous Production of Human Epidermal Growth Factor Using Escherichia coli Biofilm |
| title_full | Continuous Production of Human Epidermal Growth Factor Using Escherichia coli Biofilm |
| title_fullStr | Continuous Production of Human Epidermal Growth Factor Using Escherichia coli Biofilm |
| title_full_unstemmed | Continuous Production of Human Epidermal Growth Factor Using Escherichia coli Biofilm |
| title_short | Continuous Production of Human Epidermal Growth Factor Using Escherichia coli Biofilm |
| title_sort | continuous production of human epidermal growth factor using escherichia coli biofilm |
| topic | Escherichia coli biofilm formation hEGF secretion immobilized fermentation biofilm-based fermentation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.855059/full |
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