VCN Analysis Using Droplet Digital PCR Method in Hematopoietic Stem Cells and T Lymphocytes after Lentiviral Transduction: Optimization and Limitations

VCN Analysis Using Droplet Digital PCR Method in Hematopoietic Stem Cells and T Lymphocytes after Lentiviral Transduction: Optimization and Limitations

Background: In the context of ex vivo gene therapy or chimeric antigen receptor T cell (CAR-T) cell therapy, vector copy number (VCN) analysis in transduced cells by lentiviral vectors enables the assessment of risk and therapeutic efficiency in patients. Objectives: Here, we describe a ddPCR-b...

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Bibliographic Details
Main Authors: Aude Parcelier, Julien Buisset, Emilie Neveu, Bastien Kauffmann, Sandrine Fraboulet, Peggy Sanatine, Samia Martin, C?drick Rousseaux, Nadia Avenier, Sabine Charrier
Format: Article
Language:English
Published: Mary Ann Liebert 2022-01-01
Series:Re:GEN Open
Subjects:
lentiviral vector copy number
ddPCR
gene therapy
hematopoietic stem cells
Online Access:https://www.liebertpub.com/doi/full/10.1089/REGEN.2021.0034

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https://www.liebertpub.com/doi/full/10.1089/REGEN.2021.0034

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