Zika Virus Alters DNA Methylation of Neural Genes in an Organoid Model of the Developing Human Brain
ABSTRACT Zika virus (ZIKV) infection during early pregnancy can cause microcephaly and associated defects at birth, but whether it can induce neurologic sequelae that appear later in life remains unclear. Using a model of the developing brain based on embryonic stem cell-derived brain organoids, we...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2018-02-01
|
| Series: | mSystems |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mSystems.00219-17 |
| _version_ | 1818345545879519232 |
|---|---|
| author | Sylvie Janssens Michael Schotsaert Rahul Karnik Vinod Balasubramaniam Marion Dejosez Alexander Meissner Adolfo García-Sastre Thomas P. Zwaka |
| author_facet | Sylvie Janssens Michael Schotsaert Rahul Karnik Vinod Balasubramaniam Marion Dejosez Alexander Meissner Adolfo García-Sastre Thomas P. Zwaka |
| author_sort | Sylvie Janssens |
| collection | DOAJ |
| description | ABSTRACT Zika virus (ZIKV) infection during early pregnancy can cause microcephaly and associated defects at birth, but whether it can induce neurologic sequelae that appear later in life remains unclear. Using a model of the developing brain based on embryonic stem cell-derived brain organoids, we studied the impact of ZIKV infection on the DNA methylation pattern across the entire genome in selected neural cell types. The virus unexpectedly alters the DNA methylome of neural progenitors, astrocytes, and differentiated neurons at genes that have been implicated in the pathogenesis of a number of brain disorders, most prominently mental retardation and schizophrenia. Our results suggest that ZIKV infection during fetal development could lead to a spectrum of delayed-onset neuropsychiatric complications. IMPORTANCE Scientific research on human neural stem cells and cerebral organoids has confirmed the congenital neurotropic and neurodestructive nature of the Zika virus. However, the extent to which prenatal ZIKV infection is associated with more subtle brain alterations, such as epigenetic changes, remains ill defined. Here, we address the question of whether ZIKV infection induces DNA methylation changes with the potential to cause brain disorders later in life. |
| first_indexed | 2024-12-13T17:04:05Z |
| format | Article |
| id | doaj.art-18b89b62e6234d09b23dad5a2bc95161 |
| institution | Directory Open Access Journal |
| issn | 2379-5077 |
| language | English |
| last_indexed | 2024-12-13T17:04:05Z |
| publishDate | 2018-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSystems |
| spelling | doaj.art-18b89b62e6234d09b23dad5a2bc951612022-12-21T23:37:43ZengAmerican Society for MicrobiologymSystems2379-50772018-02-013110.1128/mSystems.00219-17Zika Virus Alters DNA Methylation of Neural Genes in an Organoid Model of the Developing Human BrainSylvie Janssens0Michael Schotsaert1Rahul Karnik2Vinod Balasubramaniam3Marion Dejosez4Alexander Meissner5Adolfo García-Sastre6Thomas P. Zwaka7Huffington Center for Cell-Based Research in Parkinson's Disease, Black Family Stem Cell Institute, Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Microbiology, Global Health and Emerging Pathogens Institute, and Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, New York, USAHarvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard University, and Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USADepartment of Microbiology, Global Health and Emerging Pathogens Institute, and Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, New York, USAHuffington Center for Cell-Based Research in Parkinson's Disease, Black Family Stem Cell Institute, Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, New York, USAHarvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard University, and Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USADepartment of Microbiology, Global Health and Emerging Pathogens Institute, and Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, New York, New York, USAHuffington Center for Cell-Based Research in Parkinson's Disease, Black Family Stem Cell Institute, Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, New York, USAABSTRACT Zika virus (ZIKV) infection during early pregnancy can cause microcephaly and associated defects at birth, but whether it can induce neurologic sequelae that appear later in life remains unclear. Using a model of the developing brain based on embryonic stem cell-derived brain organoids, we studied the impact of ZIKV infection on the DNA methylation pattern across the entire genome in selected neural cell types. The virus unexpectedly alters the DNA methylome of neural progenitors, astrocytes, and differentiated neurons at genes that have been implicated in the pathogenesis of a number of brain disorders, most prominently mental retardation and schizophrenia. Our results suggest that ZIKV infection during fetal development could lead to a spectrum of delayed-onset neuropsychiatric complications. IMPORTANCE Scientific research on human neural stem cells and cerebral organoids has confirmed the congenital neurotropic and neurodestructive nature of the Zika virus. However, the extent to which prenatal ZIKV infection is associated with more subtle brain alterations, such as epigenetic changes, remains ill defined. Here, we address the question of whether ZIKV infection induces DNA methylation changes with the potential to cause brain disorders later in life.https://journals.asm.org/doi/10.1128/mSystems.00219-17DNA methylationZika virusastrocytesbrain disordersmicrocephalyneurons |
| spellingShingle | Sylvie Janssens Michael Schotsaert Rahul Karnik Vinod Balasubramaniam Marion Dejosez Alexander Meissner Adolfo García-Sastre Thomas P. Zwaka Zika Virus Alters DNA Methylation of Neural Genes in an Organoid Model of the Developing Human Brain mSystems DNA methylation Zika virus astrocytes brain disorders microcephaly neurons |
| title | Zika Virus Alters DNA Methylation of Neural Genes in an Organoid Model of the Developing Human Brain |
| title_full | Zika Virus Alters DNA Methylation of Neural Genes in an Organoid Model of the Developing Human Brain |
| title_fullStr | Zika Virus Alters DNA Methylation of Neural Genes in an Organoid Model of the Developing Human Brain |
| title_full_unstemmed | Zika Virus Alters DNA Methylation of Neural Genes in an Organoid Model of the Developing Human Brain |
| title_short | Zika Virus Alters DNA Methylation of Neural Genes in an Organoid Model of the Developing Human Brain |
| title_sort | zika virus alters dna methylation of neural genes in an organoid model of the developing human brain |
| topic | DNA methylation Zika virus astrocytes brain disorders microcephaly neurons |
| url | https://journals.asm.org/doi/10.1128/mSystems.00219-17 |
| work_keys_str_mv | AT sylviejanssens zikavirusaltersdnamethylationofneuralgenesinanorganoidmodelofthedevelopinghumanbrain AT michaelschotsaert zikavirusaltersdnamethylationofneuralgenesinanorganoidmodelofthedevelopinghumanbrain AT rahulkarnik zikavirusaltersdnamethylationofneuralgenesinanorganoidmodelofthedevelopinghumanbrain AT vinodbalasubramaniam zikavirusaltersdnamethylationofneuralgenesinanorganoidmodelofthedevelopinghumanbrain AT mariondejosez zikavirusaltersdnamethylationofneuralgenesinanorganoidmodelofthedevelopinghumanbrain AT alexandermeissner zikavirusaltersdnamethylationofneuralgenesinanorganoidmodelofthedevelopinghumanbrain AT adolfogarciasastre zikavirusaltersdnamethylationofneuralgenesinanorganoidmodelofthedevelopinghumanbrain AT thomaspzwaka zikavirusaltersdnamethylationofneuralgenesinanorganoidmodelofthedevelopinghumanbrain |