Efficiency, Specificity and Temperature Sensitivity of Cas9 and Cas12a RNPs for DNA-free Genome Editing in Plants

Delivery of genome editing reagents using CRISPR-Cas ribonucleoproteins (RNPs) transfection offers several advantages over plasmid DNA-based delivery methods, including reduced off-target editing effects, mitigation of random integration of non-native DNA fragments, independence of vector constructi...

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Bibliographic Details
Main Authors: Raviraj Banakar, Mollie Schubert, Gavin Kurgan, Krishan Mohan Rai, Sarah F. Beaudoin, Michael A. Collingwood, Christopher A. Vakulskas, Kan Wang, Feng Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Genome Editing
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2021.760820/full