Gaseous hydrogen sulfide protects against myocardial ischemia-reperfusion injury in mice partially independent from hypometabolism.
BACKGROUND: Ischemia-reperfusion injury (IRI) is a major cause of cardiac damage following various pathological processes. Gaseous hydrogen sulfide (H2S) is protective during IRI by inducing a hypometabolic state in mice which is associated with anti-apoptotic, anti-inflammatory and antioxidant prop...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3651205?pdf=render |
| _version_ | 1811205207486365696 |
|---|---|
| author | Pauline M Snijder Rudolf A de Boer Eelke M Bos Joost C van den Born Willem-Peter T Ruifrok Inge Vreeswijk-Baudoin Marcory C R F van Dijk Jan-Luuk Hillebrands Henri G D Leuvenink Harry van Goor |
| author_facet | Pauline M Snijder Rudolf A de Boer Eelke M Bos Joost C van den Born Willem-Peter T Ruifrok Inge Vreeswijk-Baudoin Marcory C R F van Dijk Jan-Luuk Hillebrands Henri G D Leuvenink Harry van Goor |
| author_sort | Pauline M Snijder |
| collection | DOAJ |
| description | BACKGROUND: Ischemia-reperfusion injury (IRI) is a major cause of cardiac damage following various pathological processes. Gaseous hydrogen sulfide (H2S) is protective during IRI by inducing a hypometabolic state in mice which is associated with anti-apoptotic, anti-inflammatory and antioxidant properties. We investigated whether gaseous H2S administration is protective in cardiac IRI and whether non-hypometabolic concentrations of H2S have similar protective properties. METHODS: Male C57BL/6 mice received a 0, 10, or 100 ppm H2S-N2 mixture starting 30 minutes prior to ischemia until 5 minutes pre-reperfusion. IRI was inflicted by temporary ligation of the left coronary artery for 30 minutes. High-resolution respirometry equipment was used to assess CO2-production and blood pressure was measured using internal transmitters. The effects of H2S were assessed by histological and molecular analysis. RESULTS: Treatment with 100 ppm H2S decreased CO2-production by 72%, blood pressure by 14% and heart rate by 25%, while treatment with 10 ppm H2S had no effects. At day 1 of reperfusion 10 ppm H2S showed no effect on necrosis, while treatment with 100 ppm H2S reduced necrosis by 62% (p<0.05). Seven days post-reperfusion, both 10 ppm (p<0.01) and 100 ppm (p<0.05) H2S showed a reduction in fibrosis compared to IRI animals. Both 10 ppm and 100 ppm H2S reduced granulocyte-influx by 43% (p<0.05) and 60% (p<0.001), respectively. At 7 days post-reperfusion both 10 and 100 ppm H2S reduced expression of fibronectin by 63% (p<0.05) and 67% (p<0.01) and ANP by 84% and 63% (p<0.05), respectively. CONCLUSIONS: Gaseous administration of H2S is protective when administered during a cardiac ischemic insult. Although hypometabolism is restricted to small animals, we now showed that low non-hypometabolic concentrations of H2S also have protective properties in IRI. Since IRI is a frequent cause of myocardial damage during percutaneous coronary intervention and cardiac transplantation, H2S treatment might lead to novel therapeutical modalities. |
| first_indexed | 2024-04-12T03:26:50Z |
| format | Article |
| id | doaj.art-18c0bbb050d54b1dbd60a0f3f190ad9a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T03:26:50Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-18c0bbb050d54b1dbd60a0f3f190ad9a2022-12-22T03:49:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6329110.1371/journal.pone.0063291Gaseous hydrogen sulfide protects against myocardial ischemia-reperfusion injury in mice partially independent from hypometabolism.Pauline M SnijderRudolf A de BoerEelke M BosJoost C van den BornWillem-Peter T RuifrokInge Vreeswijk-BaudoinMarcory C R F van DijkJan-Luuk HillebrandsHenri G D LeuveninkHarry van GoorBACKGROUND: Ischemia-reperfusion injury (IRI) is a major cause of cardiac damage following various pathological processes. Gaseous hydrogen sulfide (H2S) is protective during IRI by inducing a hypometabolic state in mice which is associated with anti-apoptotic, anti-inflammatory and antioxidant properties. We investigated whether gaseous H2S administration is protective in cardiac IRI and whether non-hypometabolic concentrations of H2S have similar protective properties. METHODS: Male C57BL/6 mice received a 0, 10, or 100 ppm H2S-N2 mixture starting 30 minutes prior to ischemia until 5 minutes pre-reperfusion. IRI was inflicted by temporary ligation of the left coronary artery for 30 minutes. High-resolution respirometry equipment was used to assess CO2-production and blood pressure was measured using internal transmitters. The effects of H2S were assessed by histological and molecular analysis. RESULTS: Treatment with 100 ppm H2S decreased CO2-production by 72%, blood pressure by 14% and heart rate by 25%, while treatment with 10 ppm H2S had no effects. At day 1 of reperfusion 10 ppm H2S showed no effect on necrosis, while treatment with 100 ppm H2S reduced necrosis by 62% (p<0.05). Seven days post-reperfusion, both 10 ppm (p<0.01) and 100 ppm (p<0.05) H2S showed a reduction in fibrosis compared to IRI animals. Both 10 ppm and 100 ppm H2S reduced granulocyte-influx by 43% (p<0.05) and 60% (p<0.001), respectively. At 7 days post-reperfusion both 10 and 100 ppm H2S reduced expression of fibronectin by 63% (p<0.05) and 67% (p<0.01) and ANP by 84% and 63% (p<0.05), respectively. CONCLUSIONS: Gaseous administration of H2S is protective when administered during a cardiac ischemic insult. Although hypometabolism is restricted to small animals, we now showed that low non-hypometabolic concentrations of H2S also have protective properties in IRI. Since IRI is a frequent cause of myocardial damage during percutaneous coronary intervention and cardiac transplantation, H2S treatment might lead to novel therapeutical modalities.http://europepmc.org/articles/PMC3651205?pdf=render |
| spellingShingle | Pauline M Snijder Rudolf A de Boer Eelke M Bos Joost C van den Born Willem-Peter T Ruifrok Inge Vreeswijk-Baudoin Marcory C R F van Dijk Jan-Luuk Hillebrands Henri G D Leuvenink Harry van Goor Gaseous hydrogen sulfide protects against myocardial ischemia-reperfusion injury in mice partially independent from hypometabolism. PLoS ONE |
| title | Gaseous hydrogen sulfide protects against myocardial ischemia-reperfusion injury in mice partially independent from hypometabolism. |
| title_full | Gaseous hydrogen sulfide protects against myocardial ischemia-reperfusion injury in mice partially independent from hypometabolism. |
| title_fullStr | Gaseous hydrogen sulfide protects against myocardial ischemia-reperfusion injury in mice partially independent from hypometabolism. |
| title_full_unstemmed | Gaseous hydrogen sulfide protects against myocardial ischemia-reperfusion injury in mice partially independent from hypometabolism. |
| title_short | Gaseous hydrogen sulfide protects against myocardial ischemia-reperfusion injury in mice partially independent from hypometabolism. |
| title_sort | gaseous hydrogen sulfide protects against myocardial ischemia reperfusion injury in mice partially independent from hypometabolism |
| url | http://europepmc.org/articles/PMC3651205?pdf=render |
| work_keys_str_mv | AT paulinemsnijder gaseoushydrogensulfideprotectsagainstmyocardialischemiareperfusioninjuryinmicepartiallyindependentfromhypometabolism AT rudolfadeboer gaseoushydrogensulfideprotectsagainstmyocardialischemiareperfusioninjuryinmicepartiallyindependentfromhypometabolism AT eelkembos gaseoushydrogensulfideprotectsagainstmyocardialischemiareperfusioninjuryinmicepartiallyindependentfromhypometabolism AT joostcvandenborn gaseoushydrogensulfideprotectsagainstmyocardialischemiareperfusioninjuryinmicepartiallyindependentfromhypometabolism AT willempetertruifrok gaseoushydrogensulfideprotectsagainstmyocardialischemiareperfusioninjuryinmicepartiallyindependentfromhypometabolism AT ingevreeswijkbaudoin gaseoushydrogensulfideprotectsagainstmyocardialischemiareperfusioninjuryinmicepartiallyindependentfromhypometabolism AT marcorycrfvandijk gaseoushydrogensulfideprotectsagainstmyocardialischemiareperfusioninjuryinmicepartiallyindependentfromhypometabolism AT janluukhillebrands gaseoushydrogensulfideprotectsagainstmyocardialischemiareperfusioninjuryinmicepartiallyindependentfromhypometabolism AT henrigdleuvenink gaseoushydrogensulfideprotectsagainstmyocardialischemiareperfusioninjuryinmicepartiallyindependentfromhypometabolism AT harryvangoor gaseoushydrogensulfideprotectsagainstmyocardialischemiareperfusioninjuryinmicepartiallyindependentfromhypometabolism |