Loading of Cells with Fluorescent Probe to Study Intracellular Acid-base Homeostasis in Lactic Acid Bacteria
Here we describe a protocol which we have used to study the homeostasis intracellular in vivo in lactic acid bacteria (LAB) using a fluorescent probe. This type of probes can be used for determining changes in the pH of cytoplasm with high sensitivity, temporal resolution and technical simplicity as...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Bio-protocol LLC
2015-01-01
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Series: | Bio-Protocol |
Online Access: | https://bio-protocol.org/en/bpdetail?id=1380&type=0 |