Loading of Cells with Fluorescent Probe to Study Intracellular Acid-base Homeostasis in Lactic Acid Bacteria

Here we describe a protocol which we have used to study the homeostasis intracellular in vivo in lactic acid bacteria (LAB) using a fluorescent probe. This type of probes can be used for determining changes in the pH of cytoplasm with high sensitivity, temporal resolution and technical simplicity as...

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Bibliographic Details
Main Authors: Pablo Mortera, Federico Zuljan, Christian Magni, Sergio Alarcón
Format: Article
Language:English
Published: Bio-protocol LLC 2015-01-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=1380&type=0