Quantitative imaging reveals real-time Pou5f3–Nanog complexes driving dorsoventral mesendoderm patterning in zebrafish

Quantitative imaging reveals real-time Pou5f3–Nanog complexes driving dorsoventral mesendoderm patterning in zebrafish

Formation of the three embryonic germ layers is a fundamental developmental process that initiates differentiation. How the zebrafish pluripotency factor Pou5f3 (homologous to mammalian Oct4) drives lineage commitment is unclear. Here, we introduce fluorescence lifetime imaging microscopy and fluore...

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Bibliographic Details
Main Authors: Mireia Perez-Camps, Jing Tian, Serene C Chng, Kai Pin Sem, Thankiah Sudhaharan, Cathleen Teh, Malte Wachsmuth, Vladimir Korzh, Sohail Ahmed, Bruno Reversade
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2016-09-01
Series:eLife
Subjects:
transcription factor dynamics
pluripotency
gastrulation
FLIM
FCS
elabela
Online Access:https://elifesciences.org/articles/11475

Internet

https://elifesciences.org/articles/11475

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