Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold Nanoparticles
BRCA1 is the biomarker for the early diagnosis of breast cancer. Detection of BRCA1 has great significance for the genetic analysis, early diagnosis and clinical treatment of breast cancer. In this work, we developed a simple electrochemical DNA sensor based on a DNA tetrahedral-structured probe (TS...
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MDPI AG
2020-07-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/10/7/78 |
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| author | Dezhi Feng Jing Su Guifang He Yi Xu Chenguang Wang Mengmeng Zheng Qiuling Qian Xianqiang Mi |
| author_facet | Dezhi Feng Jing Su Guifang He Yi Xu Chenguang Wang Mengmeng Zheng Qiuling Qian Xianqiang Mi |
| author_sort | Dezhi Feng |
| collection | DOAJ |
| description | BRCA1 is the biomarker for the early diagnosis of breast cancer. Detection of BRCA1 has great significance for the genetic analysis, early diagnosis and clinical treatment of breast cancer. In this work, we developed a simple electrochemical DNA sensor based on a DNA tetrahedral-structured probe (TSP) and poly-adenine (polyA) mediated gold nanoparticles (AuNPs) for the sensitive detection of BRCA1. A thiol-modified TSP was used as the scaffold on the surface of the screen-printed AuNPs electrode. The capture DNA (TSP) and reporter DNA were hybridized to the target DNA (BRCA1), respectively, to form the typical sandwich system. The nanocomposites of reporter DNA (polyA at the 5′ end) combined with AuNPs were employed for signal amplification which can capture multiple enzymes by the specificity between biotin and streptavidin. Measurements were completed in the electrochemical workstation by cyclic voltammetry and amperometry and we obtained the low limit of detection of 0.1 fM with the linear range from 1 fM to 1 nM. High sensitivity and good specificity of the proposed electrochemical DNA sensor showed potential applications in clinical early diagnosis for breast cancer. |
| first_indexed | 2024-03-10T18:21:37Z |
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| id | doaj.art-18d64a881e6f4b30a46a1affd5b68b69 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T18:21:37Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
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| series | Biosensors |
| spelling | doaj.art-18d64a881e6f4b30a46a1affd5b68b692023-11-20T07:17:07ZengMDPI AGBiosensors2079-63742020-07-011077810.3390/bios10070078Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold NanoparticlesDezhi Feng0Jing Su1Guifang He2Yi Xu3Chenguang Wang4Mengmeng Zheng5Qiuling Qian6Xianqiang Mi7Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaInstitute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, ChinaBRCA1 is the biomarker for the early diagnosis of breast cancer. Detection of BRCA1 has great significance for the genetic analysis, early diagnosis and clinical treatment of breast cancer. In this work, we developed a simple electrochemical DNA sensor based on a DNA tetrahedral-structured probe (TSP) and poly-adenine (polyA) mediated gold nanoparticles (AuNPs) for the sensitive detection of BRCA1. A thiol-modified TSP was used as the scaffold on the surface of the screen-printed AuNPs electrode. The capture DNA (TSP) and reporter DNA were hybridized to the target DNA (BRCA1), respectively, to form the typical sandwich system. The nanocomposites of reporter DNA (polyA at the 5′ end) combined with AuNPs were employed for signal amplification which can capture multiple enzymes by the specificity between biotin and streptavidin. Measurements were completed in the electrochemical workstation by cyclic voltammetry and amperometry and we obtained the low limit of detection of 0.1 fM with the linear range from 1 fM to 1 nM. High sensitivity and good specificity of the proposed electrochemical DNA sensor showed potential applications in clinical early diagnosis for breast cancer.https://www.mdpi.com/2079-6374/10/7/78electrochemical DNA sensorDNA tetrahedral structured probesandwich systemBRCA1polyA-gold nanoparticles |
| spellingShingle | Dezhi Feng Jing Su Guifang He Yi Xu Chenguang Wang Mengmeng Zheng Qiuling Qian Xianqiang Mi Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold Nanoparticles Biosensors electrochemical DNA sensor DNA tetrahedral structured probe sandwich system BRCA1 polyA-gold nanoparticles |
| title | Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold Nanoparticles |
| title_full | Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold Nanoparticles |
| title_fullStr | Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold Nanoparticles |
| title_full_unstemmed | Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold Nanoparticles |
| title_short | Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold Nanoparticles |
| title_sort | electrochemical dna sensor for sensitive brca1 detection based on dna tetrahedral structured probe and poly adenine mediated gold nanoparticles |
| topic | electrochemical DNA sensor DNA tetrahedral structured probe sandwich system BRCA1 polyA-gold nanoparticles |
| url | https://www.mdpi.com/2079-6374/10/7/78 |
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