The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin
Circumferential actin belts underlying the adherens junctions of columnar epithelial cell monolayers control intercellular surface tension and cell shape to maintain tissue integrity. Yes-associated protein (YAP) and its paralog TAZ are proliferation-activating transcriptional coactivators that shut...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-08-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717309981 |
| _version_ | 1828531664074047488 |
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| author | Kana T. Furukawa Kazunari Yamashita Natsuki Sakurai Shigeo Ohno |
| author_facet | Kana T. Furukawa Kazunari Yamashita Natsuki Sakurai Shigeo Ohno |
| author_sort | Kana T. Furukawa |
| collection | DOAJ |
| description | Circumferential actin belts underlying the adherens junctions of columnar epithelial cell monolayers control intercellular surface tension and cell shape to maintain tissue integrity. Yes-associated protein (YAP) and its paralog TAZ are proliferation-activating transcriptional coactivators that shuttle between the nucleus and cytoplasm. Previous studies suggest the importance of stress fibers in the actin cytoskeleton for regulation of YAP nuclear localization; however, the role of the circumferential actin belt on YAP localization remains unclarified. By manipulating actin tension, we demonstrate that circumferential actin belt tension suppresses YAP/TAZ nuclear localization. This suppression requires Merlin, an F-actin binding protein associated with adherens junctions. Merlin physically interacts with YAP/TAZ, and nuclear export sequences of Merlin are required for suppression. Together, with the observation that the association between E-cadherin and Merlin was diminished by tension in circumferential actin belts, our results suggest that released Merlin undergoes nucleocytoplasmic shutting and mediates export of YAP/TAZ from the nucleus. |
| first_indexed | 2024-12-11T22:41:01Z |
| format | Article |
| id | doaj.art-18e91ecba4884312ab933d6ae38f1229 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-11T22:41:01Z |
| publishDate | 2017-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-18e91ecba4884312ab933d6ae38f12292022-12-22T00:47:47ZengElsevierCell Reports2211-12472017-08-012061435144710.1016/j.celrep.2017.07.032The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of MerlinKana T. Furukawa0Kazunari Yamashita1Natsuki Sakurai2Shigeo Ohno3Department of Molecular Biology, Graduate School of Medical Science, Yokohama City University, Yokohama 236-0004, JapanDepartment of Molecular Biology, Graduate School of Medical Science, Yokohama City University, Yokohama 236-0004, JapanDepartment of Molecular Biology, Graduate School of Medical Science, Yokohama City University, Yokohama 236-0004, JapanDepartment of Molecular Biology, Graduate School of Medical Science, Yokohama City University, Yokohama 236-0004, JapanCircumferential actin belts underlying the adherens junctions of columnar epithelial cell monolayers control intercellular surface tension and cell shape to maintain tissue integrity. Yes-associated protein (YAP) and its paralog TAZ are proliferation-activating transcriptional coactivators that shuttle between the nucleus and cytoplasm. Previous studies suggest the importance of stress fibers in the actin cytoskeleton for regulation of YAP nuclear localization; however, the role of the circumferential actin belt on YAP localization remains unclarified. By manipulating actin tension, we demonstrate that circumferential actin belt tension suppresses YAP/TAZ nuclear localization. This suppression requires Merlin, an F-actin binding protein associated with adherens junctions. Merlin physically interacts with YAP/TAZ, and nuclear export sequences of Merlin are required for suppression. Together, with the observation that the association between E-cadherin and Merlin was diminished by tension in circumferential actin belts, our results suggest that released Merlin undergoes nucleocytoplasmic shutting and mediates export of YAP/TAZ from the nucleus.http://www.sciencedirect.com/science/article/pii/S2211124717309981epithelial cellproliferationcircumferential actin beltYAPTAZHippoMerlinnuclear exportmechanotransduction |
| spellingShingle | Kana T. Furukawa Kazunari Yamashita Natsuki Sakurai Shigeo Ohno The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin Cell Reports epithelial cell proliferation circumferential actin belt YAP TAZ Hippo Merlin nuclear export mechanotransduction |
| title | The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin |
| title_full | The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin |
| title_fullStr | The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin |
| title_full_unstemmed | The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin |
| title_short | The Epithelial Circumferential Actin Belt Regulates YAP/TAZ through Nucleocytoplasmic Shuttling of Merlin |
| title_sort | epithelial circumferential actin belt regulates yap taz through nucleocytoplasmic shuttling of merlin |
| topic | epithelial cell proliferation circumferential actin belt YAP TAZ Hippo Merlin nuclear export mechanotransduction |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717309981 |
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