Construction of Candidate <i>Yersinia pestis</i> Vaccine Strains with Reduced Reactogenicity
The technology of directed constructing of non-reverting Y. pestis ∆lpxM mutants, lacking antibiotic resistance markers was developed. It included direct site-specific mutagenesis, cloning of mutant ∆lpxM::cat allele in pCVD442 suicide vector, homologous recombination in vivo and deletion of the ant...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2011-02-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/592 |