Isolation and purification of 3-hydroxy-3-methylglutaryl-coenzyme A by ion-exchange chromatography

For precise determination of the catalytic activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase (EC 1.1.1.34), the HMG-CoA employed as substrate must be free of HMG, CoA, and other inhibitors of HMG-CoA reductase activity. The standard purification of HMG-CoA by paper chromatography...

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Bibliographic Details
Main Authors: I P Williamson, V W Rodwell
Format: Article
Language:English
Published: Elsevier 1981-01-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520347556