Isolation and purification of 3-hydroxy-3-methylglutaryl-coenzyme A by ion-exchange chromatography
For precise determination of the catalytic activity of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase (EC 1.1.1.34), the HMG-CoA employed as substrate must be free of HMG, CoA, and other inhibitors of HMG-CoA reductase activity. The standard purification of HMG-CoA by paper chromatography...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1981-01-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520347556 |