Protein purification, crystallization, and structure determination of human DEAD-box RNA helicase DDX21 in different unwinding states

Protein purification, crystallization, and structure determination of human DEAD-box RNA helicase DDX21 in different unwinding states

Summary: RNA helicase DDX21 plays vital roles in ribosomal RNA processing and the regulation of host innate immunity during virus infection. Here, we describe the optimized protocols for nucleic acid-free protein purification and crystallization of DDX21 in its different unwinding states. Rational d...

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Bibliographic Details
Main Authors: Zijun Chen, Jinqing Huang, Jixi Li
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Molecular Biology
Protein Biochemistry
Protein expression and purification
Structural Biology
X-ray Crystallography
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005226

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http://www.sciencedirect.com/science/article/pii/S2666166722005226

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