How to find soluble proteins: a comprehensive analysis of alpha/beta hydrolases for recombinant expression in <it>E. coli</it>

<p>Abstract</p> <p>Background</p> <p>In screening of libraries derived by expression cloning, expression of active proteins in <it>E. coli </it>can be limited by formation of inclusion bodies. In these cases it would be desirable to enrich gene libraries for...

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Bibliographic Details
Main Authors: Barth Sandra, Fischer Markus, Koschorreck Markus, Pleiss Jürgen
Format: Article
Language:English
Published: BMC 2005-04-01
Series:BMC Genomics
Online Access:http://www.biomedcentral.com/1471-2164/6/49