Continuous Fluorescent Sirtuin Activity Assay Based on Fatty Acylated Lysines

Continuous Fluorescent Sirtuin Activity Assay Based on Fatty Acylated Lysines

Lysine deacetylases, like histone deacetylases (HDACs) and sirtuins (SIRTs), are involved in many regulatory processes such as control of metabolic pathways, DNA repair, and stress responses. Besides robust deacetylase activity, sirtuin isoforms SIRT2 and SIRT3 also show demyristoylase activity. Int...

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Bibliographic Details
Main Authors: Matthes Zessin, Marat Meleshin, Sebastian Hilscher, Cordelia Schiene-Fischer, Cyril Barinka, Manfred Jung, Mike Schutkowski
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:International Journal of Molecular Sciences
Subjects:
histone deacetylases
sirtuins
fluorescence quenching
sirtuin inhibitors
myristoylated substrates
continuous activity assay
Online Access:https://www.mdpi.com/1422-0067/24/8/7416

Internet

https://www.mdpi.com/1422-0067/24/8/7416

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