Development of an icIEF assay for monitoring AAV capsid proteins and application to gene therapy products

Development of an icIEF assay for monitoring AAV capsid proteins and application to gene therapy products

Adeno-associated virus (AAV) gene therapy vectors, which contain a DNA transgene packaged into a protein capsid, have shown tremendous therapeutic potential in recent years. Methods traditionally used in quality control labs, such as high-performance liquid chromatography (HPLC) and capillary electr...

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Bibliographic Details
Main Authors: Xiaoping Z. He, Thomas W. Powers, Sisi Huang, Zhenjiu Liu, Heliang Shi, John D. Orlet, Jim J. Mo, Saipraveen Srinivasan, Steven Jacobs, Kun Zhang, Herbert A. Runnels, Melissa M. Anderson, Thomas F. Lerch
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
gene therapy research
icIEF charge heterogeneity determination
AAV
deamidation
LC-MS
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050123000384

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http://www.sciencedirect.com/science/article/pii/S2329050123000384

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