Aging Additively Influences Insulin- and Insulin-Like Growth Factor-1-Mediated Endothelial Dysfunction and Antioxidant Deficiency in Spontaneously Hypertensive Rats
This study aimed to investigate the aging-related endothelial dysfunction mediated by insulin and insulin-like growth factor-1 (IGF-1) and antioxidant deficiency in hypertension. Male spontaneously hypertensive rats (SHRs) and age-matched normotensive Wistar–Kyoto rats (WKYs) were randomly divided i...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/6/676 |
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| author | Kunanya Masodsai Yi-Yuan Lin Sih-Yin Lin Chia-Ting Su Shin-Da Lee Ai-Lun Yang |
| author_facet | Kunanya Masodsai Yi-Yuan Lin Sih-Yin Lin Chia-Ting Su Shin-Da Lee Ai-Lun Yang |
| author_sort | Kunanya Masodsai |
| collection | DOAJ |
| description | This study aimed to investigate the aging-related endothelial dysfunction mediated by insulin and insulin-like growth factor-1 (IGF-1) and antioxidant deficiency in hypertension. Male spontaneously hypertensive rats (SHRs) and age-matched normotensive Wistar–Kyoto rats (WKYs) were randomly divided into 24-week-old (younger) and 48-week-old (older) groups, respectively. The endothelial function was evaluated by the insulin- and IGF-1-mediated vasorelaxation of aortic rings via the organ bath system. Serum levels of nitric oxide (NO), malondialdehyde (MDA), catalase, and total antioxidant capacity (TAC) were examined. The insulin- and IGF-1-mediated vasorelaxation was significantly impaired in both 24- and 48-week-old SHRs compared with age-matched WKYs and was significantly worse in the 48-week-old SHR than the 24-week-old SHR. After pretreatments of phosphoinositide 3-kinase (PI3K) or NO synthase (NOS) inhibitors, the insulin- and IGF-1-mediated vasorelaxation became similar among four groups. The serum level of MDA was significantly increased, while the NO, catalase, and TAC were significantly reduced in the 48-week-old SHR compared with the 24-week-old SHR. This study demonstrated that the process of aging additively affected insulin- and IGF-1-mediated endothelial dysfunction in SHRs, which could be partly attributed to the reduced NO production and antioxidant deficiency. |
| first_indexed | 2024-03-10T10:23:07Z |
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| id | doaj.art-1998b437fe2346b6908f1a8541f21431 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T10:23:07Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-1998b437fe2346b6908f1a8541f214312023-11-22T00:13:55ZengMDPI AGBiomedicines2227-90592021-06-019667610.3390/biomedicines9060676Aging Additively Influences Insulin- and Insulin-Like Growth Factor-1-Mediated Endothelial Dysfunction and Antioxidant Deficiency in Spontaneously Hypertensive RatsKunanya Masodsai0Yi-Yuan Lin1Sih-Yin Lin2Chia-Ting Su3Shin-Da Lee4Ai-Lun Yang5Faculty of Sports Science, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Exercise and Health Science, National Taipei University of Nursing and Health Sciences, Taipei 11257, TaiwanInstitute of Sports Sciences, University of Taipei, Taipei 11153, TaiwanDepartment of Occupational Therapy, College of Medicine, Fu Jen Catholic University, New Taipei City 24205, TaiwanDepartment of Physical Therapy, Asia University, Taichung 41354, TaiwanInstitute of Sports Sciences, University of Taipei, Taipei 11153, TaiwanThis study aimed to investigate the aging-related endothelial dysfunction mediated by insulin and insulin-like growth factor-1 (IGF-1) and antioxidant deficiency in hypertension. Male spontaneously hypertensive rats (SHRs) and age-matched normotensive Wistar–Kyoto rats (WKYs) were randomly divided into 24-week-old (younger) and 48-week-old (older) groups, respectively. The endothelial function was evaluated by the insulin- and IGF-1-mediated vasorelaxation of aortic rings via the organ bath system. Serum levels of nitric oxide (NO), malondialdehyde (MDA), catalase, and total antioxidant capacity (TAC) were examined. The insulin- and IGF-1-mediated vasorelaxation was significantly impaired in both 24- and 48-week-old SHRs compared with age-matched WKYs and was significantly worse in the 48-week-old SHR than the 24-week-old SHR. After pretreatments of phosphoinositide 3-kinase (PI3K) or NO synthase (NOS) inhibitors, the insulin- and IGF-1-mediated vasorelaxation became similar among four groups. The serum level of MDA was significantly increased, while the NO, catalase, and TAC were significantly reduced in the 48-week-old SHR compared with the 24-week-old SHR. This study demonstrated that the process of aging additively affected insulin- and IGF-1-mediated endothelial dysfunction in SHRs, which could be partly attributed to the reduced NO production and antioxidant deficiency.https://www.mdpi.com/2227-9059/9/6/676aginghypertensionendotheliumantioxidant activityoxidative stress |
| spellingShingle | Kunanya Masodsai Yi-Yuan Lin Sih-Yin Lin Chia-Ting Su Shin-Da Lee Ai-Lun Yang Aging Additively Influences Insulin- and Insulin-Like Growth Factor-1-Mediated Endothelial Dysfunction and Antioxidant Deficiency in Spontaneously Hypertensive Rats Biomedicines aging hypertension endothelium antioxidant activity oxidative stress |
| title | Aging Additively Influences Insulin- and Insulin-Like Growth Factor-1-Mediated Endothelial Dysfunction and Antioxidant Deficiency in Spontaneously Hypertensive Rats |
| title_full | Aging Additively Influences Insulin- and Insulin-Like Growth Factor-1-Mediated Endothelial Dysfunction and Antioxidant Deficiency in Spontaneously Hypertensive Rats |
| title_fullStr | Aging Additively Influences Insulin- and Insulin-Like Growth Factor-1-Mediated Endothelial Dysfunction and Antioxidant Deficiency in Spontaneously Hypertensive Rats |
| title_full_unstemmed | Aging Additively Influences Insulin- and Insulin-Like Growth Factor-1-Mediated Endothelial Dysfunction and Antioxidant Deficiency in Spontaneously Hypertensive Rats |
| title_short | Aging Additively Influences Insulin- and Insulin-Like Growth Factor-1-Mediated Endothelial Dysfunction and Antioxidant Deficiency in Spontaneously Hypertensive Rats |
| title_sort | aging additively influences insulin and insulin like growth factor 1 mediated endothelial dysfunction and antioxidant deficiency in spontaneously hypertensive rats |
| topic | aging hypertension endothelium antioxidant activity oxidative stress |
| url | https://www.mdpi.com/2227-9059/9/6/676 |
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