PCR/RFLP Assay for Copy Number of Mutant and Wild-Type Alleles
A PCR method for quantitating the copy number of mutant vs. wild-type alleles in DNA from cell lines is described. The assay can be used to detect a point mutation in any gene that creates or destroys a restriction site. The alleles of interest are amplified by nested PCR and labeled in a second rou...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-12-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96216st02 |