Koumine supplementation positively affects growth, circadian rhythms, and stress levels in Cyprinus carpio
This study evaluated the effects of koumine on growth performance, brain transcriptional response, expression of genes associated with circadian rhythms, and physiological stress indicators in carp (5.21 ± 0.05 g). Fish were fed four different koumine levels (0, 0.2, 2, and 20 mg/kg) of diet (crude...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423003009 |
| _version_ | 1797388793239568384 |
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| author | Dongjie Wang Bin Ye Qiujie Wang Jiayang He Zhiheng Zuo Xiang Li Jixing Zou Shaolin Xie |
| author_facet | Dongjie Wang Bin Ye Qiujie Wang Jiayang He Zhiheng Zuo Xiang Li Jixing Zou Shaolin Xie |
| author_sort | Dongjie Wang |
| collection | DOAJ |
| description | This study evaluated the effects of koumine on growth performance, brain transcriptional response, expression of genes associated with circadian rhythms, and physiological stress indicators in carp (5.21 ± 0.05 g). Fish were fed four different koumine levels (0, 0.2, 2, and 20 mg/kg) of diet (crude protein 29.71%, crude lipid 3.68%) for 10 weeks in a RAS (26.0–28.0 ℃, pH 7.3–7.5, and DO 5.5–6.1 mg/L). Each type of feed was administered to three replicate tanks (50 L, 12 cycles per day), each containing 30 fish, with feeding occurring twice daily. The results showed that koumine supplementation improved the growth performance of carp. Significant enrichment of circadian rhythm, memory, and behavior-related pathways in the brain of the koumine-supplemented group of fish. The circadian rhythm positive feedback-related genes MKP5, ATP1A, ATP2A, and CRY2 were significantly upregulated (P < 0.05), and the negative feedback-related genes REV-ERBA were significantly downregulated (P < 0.05) in the koumine supplementation group. The fish in the koumine group showed a significant increase in brain dopamine concentrations (P < 0.05) and a significant decrease in 5-hydroxytryptamine concentrations (P < 0.05). Overall, our study showed that koumine supplementation improved growth performance and reduced stress levels during rearing of carp, which may be related to the regulation of expression of circadian rhythm-related pathways. |
| first_indexed | 2024-03-08T22:45:58Z |
| format | Article |
| id | doaj.art-19a968c64ebf40c3b32f3a53bb644a49 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:45:58Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-19a968c64ebf40c3b32f3a53bb644a492023-12-17T06:39:35ZengElsevierAquaculture Reports2352-51342023-12-0133101761Koumine supplementation positively affects growth, circadian rhythms, and stress levels in Cyprinus carpioDongjie Wang0Bin Ye1Qiujie Wang2Jiayang He3Zhiheng Zuo4Xiang Li5Jixing Zou6Shaolin Xie7College of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaCollege of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaCollege of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaCollege of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, ChinaCollege of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaCanadian Food Inspection Agency, 93 Mount Edward Road, Charlottetown, PEI C1A 5T1, CanadaCollege of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, ChinaCollege of Marine Sciences, South China Agricultural University, Guangzhou, Guangdong 510642, China; Correspondence to: No. 483, Wushan Road, Wushan Street, Tianhe District, Guangzhou, Guangdong, China.This study evaluated the effects of koumine on growth performance, brain transcriptional response, expression of genes associated with circadian rhythms, and physiological stress indicators in carp (5.21 ± 0.05 g). Fish were fed four different koumine levels (0, 0.2, 2, and 20 mg/kg) of diet (crude protein 29.71%, crude lipid 3.68%) for 10 weeks in a RAS (26.0–28.0 ℃, pH 7.3–7.5, and DO 5.5–6.1 mg/L). Each type of feed was administered to three replicate tanks (50 L, 12 cycles per day), each containing 30 fish, with feeding occurring twice daily. The results showed that koumine supplementation improved the growth performance of carp. Significant enrichment of circadian rhythm, memory, and behavior-related pathways in the brain of the koumine-supplemented group of fish. The circadian rhythm positive feedback-related genes MKP5, ATP1A, ATP2A, and CRY2 were significantly upregulated (P < 0.05), and the negative feedback-related genes REV-ERBA were significantly downregulated (P < 0.05) in the koumine supplementation group. The fish in the koumine group showed a significant increase in brain dopamine concentrations (P < 0.05) and a significant decrease in 5-hydroxytryptamine concentrations (P < 0.05). Overall, our study showed that koumine supplementation improved growth performance and reduced stress levels during rearing of carp, which may be related to the regulation of expression of circadian rhythm-related pathways.http://www.sciencedirect.com/science/article/pii/S2352513423003009KoumineFish welfareGrowth performanceCircadian rhythmDopamine |
| spellingShingle | Dongjie Wang Bin Ye Qiujie Wang Jiayang He Zhiheng Zuo Xiang Li Jixing Zou Shaolin Xie Koumine supplementation positively affects growth, circadian rhythms, and stress levels in Cyprinus carpio Aquaculture Reports Koumine Fish welfare Growth performance Circadian rhythm Dopamine |
| title | Koumine supplementation positively affects growth, circadian rhythms, and stress levels in Cyprinus carpio |
| title_full | Koumine supplementation positively affects growth, circadian rhythms, and stress levels in Cyprinus carpio |
| title_fullStr | Koumine supplementation positively affects growth, circadian rhythms, and stress levels in Cyprinus carpio |
| title_full_unstemmed | Koumine supplementation positively affects growth, circadian rhythms, and stress levels in Cyprinus carpio |
| title_short | Koumine supplementation positively affects growth, circadian rhythms, and stress levels in Cyprinus carpio |
| title_sort | koumine supplementation positively affects growth circadian rhythms and stress levels in cyprinus carpio |
| topic | Koumine Fish welfare Growth performance Circadian rhythm Dopamine |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423003009 |
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