Screening of CRISPR-Cas9-generated point mutant mice using MiSeq and locked nucleic acid probe PCR

Screening of CRISPR-Cas9-generated point mutant mice using MiSeq and locked nucleic acid probe PCR

Summary: CRISPR-Cas9-mediated, site-directed mutagenesis in mice generates mosaic founder mice with varied efficiency of desired point mutation and other non-homologous end-joined variants. Here, we present a protocol for design, sample preparation, and analysis for identification of mice with the d...

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Bibliographic Details
Main Authors: Kommireddy Vasu, Paul L. Fox
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Sequencing
Model organisms
Molecular biology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004913

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http://www.sciencedirect.com/science/article/pii/S2666166721004913

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