An optimized protocol to assess SUMOylation in the plant Capsella rubella using two-component DEX-inducible transformants

An optimized protocol to assess SUMOylation in the plant Capsella rubella using two-component DEX-inducible transformants

Summary: Here, we present an efficient protocol to test the SUMOylation of a target protein in the plant Capsella rubella based on overexpression of dexamethasone (DEX)-inducible tagged proteins. We describe the construction of two-component, FLAG-tagged DEX-inducible plasmids. We then detail the tr...

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Bibliographic Details
Main Authors: Yang Dong, Zhi-Cheng Hu, Lars Østergaard
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Model Organisms
Plant sciences
Molecular Biology
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722000776

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http://www.sciencedirect.com/science/article/pii/S2666166722000776

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