Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats
Introduction: Gonadotropin-inhibitory hormone (GnIH) and its mammalian orthologue RF amide-related peptide (RFRP) are known to inhibit the secretion of gonadotropins. In addition, substance P (SP), a member of tachykinin’s family, can increase the firing rate of kisspeptin/ neurokinin B/dynorphin (K...
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| Format: | Article |
| Language: | English |
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Zabol University of Medical sciences
2018-09-01
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| Series: | International Journal of Basic Science in Medicine |
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| Online Access: | http://ijbsm.zbmu.ac.ir/PDF/ijbsm-3254 |
| _version_ | 1818144631208017920 |
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| author | Parastoo Rahdar Homayoun Khazali |
| author_facet | Parastoo Rahdar Homayoun Khazali |
| author_sort | Parastoo Rahdar |
| collection | DOAJ |
| description | Introduction: Gonadotropin-inhibitory hormone (GnIH) and its mammalian orthologue RF amide-related peptide (RFRP) are known to inhibit the secretion of gonadotropins. In addition, substance P (SP), a member of tachykinin’s family, can increase the firing rate of kisspeptin/ neurokinin B/dynorphin (KNDy) neurons and provoke the secretion of gonadotropins. In this experimental study, we investigated the effects of co-administration of RFRP-3 and SP on the expression of KISS-1 and GnRH genes, as gonadotropin regulator genes, in male rats. Methods: Forty-two mature Wistar rats were randomly allocated into 7 groups (n=6 in each group). Animals in each group intracerebroventricularly received either saline+DMSO, SP (1nmol), RFRP-3 (5 nmol), SP (1nmol) + RFRP-3 (5 nmol), SP (1 nmol) + RF9 (RFRP-3 receptor antagonist, 10 nmol), SP (1 nmol) + P234 (kisspeptin receptor antagonist, 1 nmol) + RFRP-3 (5 nmol), or SP (1 nmol) + CP-96,345 (SP receptor antagonist, 5 nmol) + RFRP-3 (5 nmol). Two hours after injections, hypothalamic samples were collected to evaluate the expression of target genes by real-time PCR. Results: Injections in SP and SP + RF9 groups increased the expression of both GnRH and KISS-1 genes (P<0.05). Injections in RFRP-3 and SP + RFRP-3 + CP-96,345 groups significantly decreased the expression of GnRH and KISS-1 genes (P<0.05). However, injections of SP + RFRP-3 and SP + RFRP-3 + P234 did not significantly change the expression of GnRH and KISS1 genes. Conclusion: The results of our study indicated that SP antagonized the effects of RFRP-3 in the expression of hypothalamic KISS-1 and GnRH genes. |
| first_indexed | 2024-12-11T11:50:38Z |
| format | Article |
| id | doaj.art-19c54e4f0ead45fcaa08095aef983852 |
| institution | Directory Open Access Journal |
| issn | 2476-664X |
| language | English |
| last_indexed | 2024-12-11T11:50:38Z |
| publishDate | 2018-09-01 |
| publisher | Zabol University of Medical sciences |
| record_format | Article |
| series | International Journal of Basic Science in Medicine |
| spelling | doaj.art-19c54e4f0ead45fcaa08095aef9838522022-12-22T01:08:22ZengZabol University of Medical sciencesInternational Journal of Basic Science in Medicine2476-664X2018-09-013312713210.15171/ijbsm.2018.23ijbsm-3254Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar RatsParastoo Rahdar0Homayoun Khazali1Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, IranDepartment of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, IranIntroduction: Gonadotropin-inhibitory hormone (GnIH) and its mammalian orthologue RF amide-related peptide (RFRP) are known to inhibit the secretion of gonadotropins. In addition, substance P (SP), a member of tachykinin’s family, can increase the firing rate of kisspeptin/ neurokinin B/dynorphin (KNDy) neurons and provoke the secretion of gonadotropins. In this experimental study, we investigated the effects of co-administration of RFRP-3 and SP on the expression of KISS-1 and GnRH genes, as gonadotropin regulator genes, in male rats. Methods: Forty-two mature Wistar rats were randomly allocated into 7 groups (n=6 in each group). Animals in each group intracerebroventricularly received either saline+DMSO, SP (1nmol), RFRP-3 (5 nmol), SP (1nmol) + RFRP-3 (5 nmol), SP (1 nmol) + RF9 (RFRP-3 receptor antagonist, 10 nmol), SP (1 nmol) + P234 (kisspeptin receptor antagonist, 1 nmol) + RFRP-3 (5 nmol), or SP (1 nmol) + CP-96,345 (SP receptor antagonist, 5 nmol) + RFRP-3 (5 nmol). Two hours after injections, hypothalamic samples were collected to evaluate the expression of target genes by real-time PCR. Results: Injections in SP and SP + RF9 groups increased the expression of both GnRH and KISS-1 genes (P<0.05). Injections in RFRP-3 and SP + RFRP-3 + CP-96,345 groups significantly decreased the expression of GnRH and KISS-1 genes (P<0.05). However, injections of SP + RFRP-3 and SP + RFRP-3 + P234 did not significantly change the expression of GnRH and KISS1 genes. Conclusion: The results of our study indicated that SP antagonized the effects of RFRP-3 in the expression of hypothalamic KISS-1 and GnRH genes.http://ijbsm.zbmu.ac.ir/PDF/ijbsm-3254RF amid-related peptid-3Substance PGnRHKISS-1Rat |
| spellingShingle | Parastoo Rahdar Homayoun Khazali Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats International Journal of Basic Science in Medicine RF amid-related peptid-3 Substance P GnRH KISS-1 Rat |
| title | Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats |
| title_full | Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats |
| title_fullStr | Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats |
| title_full_unstemmed | Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats |
| title_short | Central Injection of Substance P Antagonizes the RF Amide-Related Peptide-3 Impacts on Hypothalamic KISS-1 and GnRH Gene Expressions in Male Wistar Rats |
| title_sort | central injection of substance p antagonizes the rf amide related peptide 3 impacts on hypothalamic kiss 1 and gnrh gene expressions in male wistar rats |
| topic | RF amid-related peptid-3 Substance P GnRH KISS-1 Rat |
| url | http://ijbsm.zbmu.ac.ir/PDF/ijbsm-3254 |
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