Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry
Control of cell size requires molecular size sensors that are coupled to the cell cycle. Rod-shaped fission yeast cells divide at a threshold size partly due to Cdr2 kinase, which forms nodes at the medial cell cortex where it inhibits the Cdk1-inhibitor Wee1. Pom1 kinase phosphorylates and inhibits...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-05-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/46003 |
| _version_ | 1811199795414433792 |
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| author | Corey A H Allard Hannah E Opalko James B Moseley |
| author_facet | Corey A H Allard Hannah E Opalko James B Moseley |
| author_sort | Corey A H Allard |
| collection | DOAJ |
| description | Control of cell size requires molecular size sensors that are coupled to the cell cycle. Rod-shaped fission yeast cells divide at a threshold size partly due to Cdr2 kinase, which forms nodes at the medial cell cortex where it inhibits the Cdk1-inhibitor Wee1. Pom1 kinase phosphorylates and inhibits Cdr2, and forms cortical concentration gradients from cell poles. Pom1 inhibits Cdr2 signaling to Wee1 specifically in small cells, but the time and place of their regulatory interactions were unclear. We show that Pom1 forms stable oligomeric clusters that dynamically sample the cell cortex. Binding frequency is patterned into a concentration gradient by the polarity landmarks Tea1 and Tea4. Pom1 clusters colocalize with Cdr2 nodes, forming a glucose-modulated inhibitory threshold against node activation. Our work reveals how Pom1-Cdr2-Wee1 operates in multiprotein clusters at the cortex to promote mitotic entry at a cell size that can be modified by nutrient availability. |
| first_indexed | 2024-04-12T01:53:26Z |
| format | Article |
| id | doaj.art-19df48cfe611405fa29edd29431fa3ff |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:53:26Z |
| publishDate | 2019-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-19df48cfe611405fa29edd29431fa3ff2022-12-22T03:52:51ZengeLife Sciences Publications LtdeLife2050-084X2019-05-01810.7554/eLife.46003Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entryCorey A H Allard0Hannah E Opalko1James B Moseley2https://orcid.org/0000-0002-7354-7416Department of Biochemistry and Cell Biology, The Geisel School of Medicine at Dartmouth, Hanover, United StatesDepartment of Biochemistry and Cell Biology, The Geisel School of Medicine at Dartmouth, Hanover, United StatesDepartment of Biochemistry and Cell Biology, The Geisel School of Medicine at Dartmouth, Hanover, United StatesControl of cell size requires molecular size sensors that are coupled to the cell cycle. Rod-shaped fission yeast cells divide at a threshold size partly due to Cdr2 kinase, which forms nodes at the medial cell cortex where it inhibits the Cdk1-inhibitor Wee1. Pom1 kinase phosphorylates and inhibits Cdr2, and forms cortical concentration gradients from cell poles. Pom1 inhibits Cdr2 signaling to Wee1 specifically in small cells, but the time and place of their regulatory interactions were unclear. We show that Pom1 forms stable oligomeric clusters that dynamically sample the cell cortex. Binding frequency is patterned into a concentration gradient by the polarity landmarks Tea1 and Tea4. Pom1 clusters colocalize with Cdr2 nodes, forming a glucose-modulated inhibitory threshold against node activation. Our work reveals how Pom1-Cdr2-Wee1 operates in multiprotein clusters at the cortex to promote mitotic entry at a cell size that can be modified by nutrient availability.https://elifesciences.org/articles/46003yeastcell cyclepombekinasesizecdr2 |
| spellingShingle | Corey A H Allard Hannah E Opalko James B Moseley Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry eLife yeast cell cycle pombe kinase size cdr2 |
| title | Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry |
| title_full | Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry |
| title_fullStr | Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry |
| title_full_unstemmed | Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry |
| title_short | Stable Pom1 clusters form a glucose-modulated concentration gradient that regulates mitotic entry |
| title_sort | stable pom1 clusters form a glucose modulated concentration gradient that regulates mitotic entry |
| topic | yeast cell cycle pombe kinase size cdr2 |
| url | https://elifesciences.org/articles/46003 |
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