A Novel Method to Reduce ELISA Serial Dilution Assay Workload Applied to SARS-CoV-2 and Seasonal HCoVs

A Novel Method to Reduce ELISA Serial Dilution Assay Workload Applied to SARS-CoV-2 and Seasonal HCoVs

Assays using ELISA measurements on serially diluted serum samples have been heavily used to measure serum reactivity to SARS-CoV-2 antigens and are widely used in virology and elsewhere in biology. We test a method using Bayesian hierarchical modelling to reduce the workload of these assays and meas...

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Bibliographic Details
Main Authors: David Pattinson, Peter Jester, Lizheng Guan, Seiya Yamayoshi, Shiho Chiba, Robert Presler, Hongyu Rao, Kiyoko Iwatsuki-Horimoto, Nobuhiro Ikeda, Masao Hagihara, Tomoyuki Uchida, Keiko Mitamura, Peter Halfmann, Gabriele Neumann, Yoshihiro Kawaoka
Format: Article
Language:English
Published: MDPI AG 2022-03-01
Series:Viruses
Subjects:
SARS-CoV-2
HCoV-OC43
HCoV-HKU1
HCoV-229E
HCoV-NL63
ELISA
Online Access:https://www.mdpi.com/1999-4915/14/3/562

Internet

https://www.mdpi.com/1999-4915/14/3/562

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