Ultra-sensitive digital quantification of proteins and mRNA in single cells
Digital proximity ligation assay (dPLA) can measure proteins and mRNAs in single cells, but is not compatible with cell imaging and cannot quantify rare proteins due to a high dilution factor. Here the authors present an automated microfluidic device that combines live-cell imaging, chemical stimula...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2019-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-11531-z |