Ultra-sensitive digital quantification of proteins and mRNA in single cells
Digital proximity ligation assay (dPLA) can measure proteins and mRNAs in single cells, but is not compatible with cell imaging and cannot quantify rare proteins due to a high dilution factor. Here the authors present an automated microfluidic device that combines live-cell imaging, chemical stimula...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2019-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-11531-z |
| _version_ | 1828970100594573312 |
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| author | Jing Lin Christian Jordi Minjun Son Hoang Van Phan Nir Drayman Mustafa Fatih Abasiyanik Luke Vistain Hsiung-Lin Tu Savaş Tay |
| author_facet | Jing Lin Christian Jordi Minjun Son Hoang Van Phan Nir Drayman Mustafa Fatih Abasiyanik Luke Vistain Hsiung-Lin Tu Savaş Tay |
| author_sort | Jing Lin |
| collection | DOAJ |
| description | Digital proximity ligation assay (dPLA) can measure proteins and mRNAs in single cells, but is not compatible with cell imaging and cannot quantify rare proteins due to a high dilution factor. Here the authors present an automated microfluidic device that combines live-cell imaging, chemical stimulation, and dPLA in a smaller reaction volume. |
| first_indexed | 2024-12-14T12:44:54Z |
| format | Article |
| id | doaj.art-1a00989204844592904106524e044438 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-14T12:44:54Z |
| publishDate | 2019-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-1a00989204844592904106524e0444382022-12-21T23:00:49ZengNature PortfolioNature Communications2041-17232019-08-0110111010.1038/s41467-019-11531-zUltra-sensitive digital quantification of proteins and mRNA in single cellsJing Lin0Christian Jordi1Minjun Son2Hoang Van Phan3Nir Drayman4Mustafa Fatih Abasiyanik5Luke Vistain6Hsiung-Lin Tu7Savaş Tay8Pritzker School of Molecular Engineering, University of ChicagoPritzker School of Molecular Engineering, University of ChicagoPritzker School of Molecular Engineering, University of ChicagoPritzker School of Molecular Engineering, University of ChicagoPritzker School of Molecular Engineering, University of ChicagoPritzker School of Molecular Engineering, University of ChicagoPritzker School of Molecular Engineering, University of ChicagoPritzker School of Molecular Engineering, University of ChicagoPritzker School of Molecular Engineering, University of ChicagoDigital proximity ligation assay (dPLA) can measure proteins and mRNAs in single cells, but is not compatible with cell imaging and cannot quantify rare proteins due to a high dilution factor. Here the authors present an automated microfluidic device that combines live-cell imaging, chemical stimulation, and dPLA in a smaller reaction volume.https://doi.org/10.1038/s41467-019-11531-z |
| spellingShingle | Jing Lin Christian Jordi Minjun Son Hoang Van Phan Nir Drayman Mustafa Fatih Abasiyanik Luke Vistain Hsiung-Lin Tu Savaş Tay Ultra-sensitive digital quantification of proteins and mRNA in single cells Nature Communications |
| title | Ultra-sensitive digital quantification of proteins and mRNA in single cells |
| title_full | Ultra-sensitive digital quantification of proteins and mRNA in single cells |
| title_fullStr | Ultra-sensitive digital quantification of proteins and mRNA in single cells |
| title_full_unstemmed | Ultra-sensitive digital quantification of proteins and mRNA in single cells |
| title_short | Ultra-sensitive digital quantification of proteins and mRNA in single cells |
| title_sort | ultra sensitive digital quantification of proteins and mrna in single cells |
| url | https://doi.org/10.1038/s41467-019-11531-z |
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