Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates
Abstract Cold storage of platelet concentrates (PC) has become attractive due to the reduced risk of bacterial proliferation, but in vivo circulation time of cold-stored platelets is reduced. Ca2+ release from storage organelles and higher activity of Ca2+ pumps at temperatures < 15 °C triggers c...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-10231-x |
| _version_ | 1818274159716728832 |
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| author | Konstanze Aurich Jan Wesche Martin Ulbricht Oliver Otto Andreas Greinacher Raghavendra Palankar |
| author_facet | Konstanze Aurich Jan Wesche Martin Ulbricht Oliver Otto Andreas Greinacher Raghavendra Palankar |
| author_sort | Konstanze Aurich |
| collection | DOAJ |
| description | Abstract Cold storage of platelet concentrates (PC) has become attractive due to the reduced risk of bacterial proliferation, but in vivo circulation time of cold-stored platelets is reduced. Ca2+ release from storage organelles and higher activity of Ca2+ pumps at temperatures < 15 °C triggers cytoskeleton changes. This is suppressed by Mg2+ addition, avoiding a shift in Ca2+ hemostasis and cytoskeletal alterations. We report on the impact of 2–10 mM Mg2+ on cytoskeleton alterations of platelets from PC stored at room temperature (RT) or 4 °C in additive solution (PAS), 30% plasma. Deformation of platelets was assessed by real-time deformability cytometry (RT-DC), a method for biomechanical cell characterization. Deformation was strongly affected by storage at 4 °C and preserved by Mg2+ addition ≥ 4 mM Mg2+ (mean ± SD of median deformation 4 °C vs. 4 °C + 10 mM Mg2+ 0.073 ± 0.021 vs. 0.118 ± 0.023, p < 0.01; n = 6, day 7). These results were confirmed by immunofluorescence microscopy, showing that Mg2+ ≥ 4 mM prevents 4 °C storage induced cytoskeletal structure lesion. Standard in vitro platelet function tests showed minor differences between RT and cold-stored platelets. Hypotonic shock response was not significantly different between RT stored (56.38 ± 29.36%) and cold-stored platelets with (55.22 ± 11.16%) or without magnesium (45.65 ± 11.59%; p = 0.042, all n = 6, day 1). CD62P expression and platelet aggregation response were similar between RT and 4 °C stored platelets, with minor changes in the presence of higher Mg2+ concentrations. In conclusion, increasing Mg2+ up to 10 mM in PAS counteracts 4 °C storage lesions in platelets, maintains platelet cytoskeletal integrity and biomechanical properties comparable to RT stored platelets. |
| first_indexed | 2024-12-12T22:09:26Z |
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| id | doaj.art-1a137f41e5554d4eaafe150da5d0d5d5 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T22:09:26Z |
| publishDate | 2022-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1a137f41e5554d4eaafe150da5d0d5d52022-12-22T00:10:17ZengNature PortfolioScientific Reports2045-23222022-04-011211910.1038/s41598-022-10231-xDivalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentratesKonstanze Aurich0Jan Wesche1Martin Ulbricht2Oliver Otto3Andreas Greinacher4Raghavendra Palankar5Institut für Transfusionsmedizin, Universitätsmedizin GreifswaldInstitut für Transfusionsmedizin, Universitätsmedizin GreifswaldInstitut für Transfusionsmedizin, Universitätsmedizin GreifswaldZentrum Für Innovationskompetenz: Humorale Immunreaktionen Bei Kardiovaskulären Erkrankungen, Universität GreifswaldInstitut für Transfusionsmedizin, Universitätsmedizin GreifswaldInstitut für Transfusionsmedizin, Universitätsmedizin GreifswaldAbstract Cold storage of platelet concentrates (PC) has become attractive due to the reduced risk of bacterial proliferation, but in vivo circulation time of cold-stored platelets is reduced. Ca2+ release from storage organelles and higher activity of Ca2+ pumps at temperatures < 15 °C triggers cytoskeleton changes. This is suppressed by Mg2+ addition, avoiding a shift in Ca2+ hemostasis and cytoskeletal alterations. We report on the impact of 2–10 mM Mg2+ on cytoskeleton alterations of platelets from PC stored at room temperature (RT) or 4 °C in additive solution (PAS), 30% plasma. Deformation of platelets was assessed by real-time deformability cytometry (RT-DC), a method for biomechanical cell characterization. Deformation was strongly affected by storage at 4 °C and preserved by Mg2+ addition ≥ 4 mM Mg2+ (mean ± SD of median deformation 4 °C vs. 4 °C + 10 mM Mg2+ 0.073 ± 0.021 vs. 0.118 ± 0.023, p < 0.01; n = 6, day 7). These results were confirmed by immunofluorescence microscopy, showing that Mg2+ ≥ 4 mM prevents 4 °C storage induced cytoskeletal structure lesion. Standard in vitro platelet function tests showed minor differences between RT and cold-stored platelets. Hypotonic shock response was not significantly different between RT stored (56.38 ± 29.36%) and cold-stored platelets with (55.22 ± 11.16%) or without magnesium (45.65 ± 11.59%; p = 0.042, all n = 6, day 1). CD62P expression and platelet aggregation response were similar between RT and 4 °C stored platelets, with minor changes in the presence of higher Mg2+ concentrations. In conclusion, increasing Mg2+ up to 10 mM in PAS counteracts 4 °C storage lesions in platelets, maintains platelet cytoskeletal integrity and biomechanical properties comparable to RT stored platelets.https://doi.org/10.1038/s41598-022-10231-x |
| spellingShingle | Konstanze Aurich Jan Wesche Martin Ulbricht Oliver Otto Andreas Greinacher Raghavendra Palankar Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates Scientific Reports |
| title | Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates |
| title_full | Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates |
| title_fullStr | Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates |
| title_full_unstemmed | Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates |
| title_short | Divalent magnesium restores cytoskeletal storage lesions in cold-stored platelet concentrates |
| title_sort | divalent magnesium restores cytoskeletal storage lesions in cold stored platelet concentrates |
| url | https://doi.org/10.1038/s41598-022-10231-x |
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