Simultaneous Determination of Konjac Glucomannan and Starch in Konjac Flour by High Performance Liquid Chromatography
This research aims to establish an HPLC method for the simultaneous determination of konjac glucomannan (KGM) and starch in konjac powder. In this paper, konjac powder was hydrolyzed into glucose and mannose by acid hydrolysis. The hydrolysate of konjac powder was derivatized by 1-phenyl-3-methyl-5-...
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| Format: | Article |
| Language: | English |
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Academy of National Food and Strategic Reserves Administration
2022-07-01
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| Series: | Liang you shipin ke-ji |
| Subjects: | |
| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220426?st=article_issue |
| _version_ | 1797869454826143744 |
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| author | WU Bo LI Ming-xia LIU Shu-rui LIU Zhong-si HUANG Chao-bo CHEN Li-shui |
| author_facet | WU Bo LI Ming-xia LIU Shu-rui LIU Zhong-si HUANG Chao-bo CHEN Li-shui |
| author_sort | WU Bo |
| collection | DOAJ |
| description | This research aims to establish an HPLC method for the simultaneous determination of konjac glucomannan (KGM) and starch in konjac powder. In this paper, konjac powder was hydrolyzed into glucose and mannose by acid hydrolysis. The hydrolysate of konjac powder was derivatized by 1-phenyl-3-methyl-5- pyrazolone (PMP). The contents of glucomannan (KGM) and starch in konjac powder were determined by HPLC with UV detector. The relative standard deviations (RSD) of KGM and starch in four kinds of konjac flour were ≤4.40%, the recovery of glucomannan was 87.98%~103.31%, and the RSD was ≤5.25%; The recovery of starch was 91.50%~108.05%, RSD≤7.67%. This method can be used for the simultaneous determination of KGM and starch in konjac powder. The establishment of this method is of great significance to the classification control of konjac powder. |
| first_indexed | 2024-04-10T00:12:53Z |
| format | Article |
| id | doaj.art-1a1b76cbe2004478b7c8778a6ed98ce8 |
| institution | Directory Open Access Journal |
| issn | 1007-7561 |
| language | English |
| last_indexed | 2024-04-10T00:12:53Z |
| publishDate | 2022-07-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
| record_format | Article |
| series | Liang you shipin ke-ji |
| spelling | doaj.art-1a1b76cbe2004478b7c8778a6ed98ce82023-03-16T07:20:12ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612022-07-0130417217710.16210/j.cnki.1007-7561.2022.04.023Simultaneous Determination of Konjac Glucomannan and Starch in Konjac Flour by High Performance Liquid ChromatographyWU Bo0LI Ming-xia1LIU Shu-rui2LIU Zhong-si3HUANG Chao-bo4CHEN Li-shui5Weilong Biotechnology Limited Company, Luohe Key Laboratory of Food Safety and Nutritional Health, Luohe, Henan 462000, ChinaWeilong Biotechnology Limited Company, Luohe Key Laboratory of Food Safety and Nutritional Health, Luohe, Henan 462000, ChinaWeilong Biotechnology Limited Company, Luohe Key Laboratory of Food Safety and Nutritional Health, Luohe, Henan 462000, ChinaWeilong Biotechnology Limited Company, Luohe Key Laboratory of Food Safety and Nutritional Health, Luohe, Henan 462000, ChinaWeilong Biotechnology Limited Company, Luohe Key Laboratory of Food Safety and Nutritional Health, Luohe, Henan 462000, ChinaWeilong Biotechnology Limited Company, Luohe Key Laboratory of Food Safety and Nutritional Health, Luohe, Henan 462000, ChinaThis research aims to establish an HPLC method for the simultaneous determination of konjac glucomannan (KGM) and starch in konjac powder. In this paper, konjac powder was hydrolyzed into glucose and mannose by acid hydrolysis. The hydrolysate of konjac powder was derivatized by 1-phenyl-3-methyl-5- pyrazolone (PMP). The contents of glucomannan (KGM) and starch in konjac powder were determined by HPLC with UV detector. The relative standard deviations (RSD) of KGM and starch in four kinds of konjac flour were ≤4.40%, the recovery of glucomannan was 87.98%~103.31%, and the RSD was ≤5.25%; The recovery of starch was 91.50%~108.05%, RSD≤7.67%. This method can be used for the simultaneous determination of KGM and starch in konjac powder. The establishment of this method is of great significance to the classification control of konjac powder.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220426?st=article_issuekonjac glucomannanstarchpre-column derivatizationhigh performance liquid chromatography |
| spellingShingle | WU Bo LI Ming-xia LIU Shu-rui LIU Zhong-si HUANG Chao-bo CHEN Li-shui Simultaneous Determination of Konjac Glucomannan and Starch in Konjac Flour by High Performance Liquid Chromatography Liang you shipin ke-ji konjac glucomannan starch pre-column derivatization high performance liquid chromatography |
| title | Simultaneous Determination of Konjac Glucomannan and Starch in Konjac Flour by High Performance Liquid Chromatography |
| title_full | Simultaneous Determination of Konjac Glucomannan and Starch in Konjac Flour by High Performance Liquid Chromatography |
| title_fullStr | Simultaneous Determination of Konjac Glucomannan and Starch in Konjac Flour by High Performance Liquid Chromatography |
| title_full_unstemmed | Simultaneous Determination of Konjac Glucomannan and Starch in Konjac Flour by High Performance Liquid Chromatography |
| title_short | Simultaneous Determination of Konjac Glucomannan and Starch in Konjac Flour by High Performance Liquid Chromatography |
| title_sort | simultaneous determination of konjac glucomannan and starch in konjac flour by high performance liquid chromatography |
| topic | konjac glucomannan starch pre-column derivatization high performance liquid chromatography |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220426?st=article_issue |
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