Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup

Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup

Summary: Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis. We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes. Using a stopped-flow setup, AQP embedded in proteoliposomes...

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Bibliographic Details
Main Authors: Jonas Hyld Steffen, Julie Winkel Missel, Tamim Al-Jubair, Philip Kitchen, Mootaz M. Salman, Roslyn M. Bill, Susanna Törnroth-Horsefield, Pontus Gourdon
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Single-molecule Assays
Cell Membrane
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722001927

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http://www.sciencedirect.com/science/article/pii/S2666166722001927

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