Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense
Myricetin, a polyhydroxyflavone compound, is one of the main ingredients of various human foods and therefore also known as dietary flavonoids. Due to the continuous emergence of resistant strains of herpesviruses, novel control measures are required. In the present study, myricetin exhibited potent...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.985108/full |
| _version_ | 1798033826225586176 |
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| author | Huaiyue Hu Zhiqiang Hu Yingying Zhang Hongping Wan Zhongqiong Yin Lixia Li Xiaoxia Liang Xinghong Zhao Lizi Yin Gang Ye Yuan-Feng Zou Huaqiao Tang Renyong Jia Yaqin Chen Hao Zhou Hao Zhou Xu Song |
| author_facet | Huaiyue Hu Zhiqiang Hu Yingying Zhang Hongping Wan Zhongqiong Yin Lixia Li Xiaoxia Liang Xinghong Zhao Lizi Yin Gang Ye Yuan-Feng Zou Huaqiao Tang Renyong Jia Yaqin Chen Hao Zhou Hao Zhou Xu Song |
| author_sort | Huaiyue Hu |
| collection | DOAJ |
| description | Myricetin, a polyhydroxyflavone compound, is one of the main ingredients of various human foods and therefore also known as dietary flavonoids. Due to the continuous emergence of resistant strains of herpesviruses, novel control measures are required. In the present study, myricetin exhibited potent antiviral activity against pseudorabies virus (PRV), a model organism of herpesvirus. The suppression rate could reach up to 96.4% at a concentration of 500 μM in cells, and the 50% inhibitory concentration (IC50) was 42.69 μM. Moreover, the inhibitory activity was not attenuated by the increased amount of infective dose, and a significant reduction of intracellular PRV virions was observed by indirect immunofluorescence. A mode of action study indicated that myricetin could directly inactivate the virus in vitro, leading to inhibition of viral adsorption, penetration and replication in cells. In addition to direct killing effect, myricetin could also activate host antiviral defense through regulation of apoptosis-related gene expressions (Bcl-2, Bcl-xl, Bax), NF-κB and MAPK signaling pathways and cytokine gene expressions (IL-1α, IL-1β, IL-6, c-Jun, STAT1, c-Fos, and c-Myc). In PRV-infected mouse model, myricetin could enhance the survival rate by 40% at 5 days post infection, and viral loads in kidney, liver, lung, spleen, and brain were significantly decreased. The pathological changes caused by PRV infection were improved by myricetin treatment. The gene expressions of inflammatory factors (MCP-1, G-CSF, IL-1α, IL-1β, and IL-6) and apoptotic factors (Bcl-xl, Bcl-2, and Bax) were regulated by myricetin in PRV-infected mice. The present findings suggest that myricetin can effectively inhibit PRV infection and become a candidate for development of new anti-herpesvirus drugs. |
| first_indexed | 2024-04-11T20:35:49Z |
| format | Article |
| id | doaj.art-1a470fc198574ef9a0f2951b7f808c76 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T20:35:49Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1a470fc198574ef9a0f2951b7f808c762022-12-22T04:04:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.985108985108Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defenseHuaiyue Hu0Zhiqiang Hu1Yingying Zhang2Hongping Wan3Zhongqiong Yin4Lixia Li5Xiaoxia Liang6Xinghong Zhao7Lizi Yin8Gang Ye9Yuan-Feng Zou10Huaqiao Tang11Renyong Jia12Yaqin Chen13Hao Zhou14Hao Zhou15Xu Song16Natural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaShandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd., Dezhou, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Microbiology, NYU Grossman School of Medicine, New York, NY, United StatesNatural Medicine Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaMyricetin, a polyhydroxyflavone compound, is one of the main ingredients of various human foods and therefore also known as dietary flavonoids. Due to the continuous emergence of resistant strains of herpesviruses, novel control measures are required. In the present study, myricetin exhibited potent antiviral activity against pseudorabies virus (PRV), a model organism of herpesvirus. The suppression rate could reach up to 96.4% at a concentration of 500 μM in cells, and the 50% inhibitory concentration (IC50) was 42.69 μM. Moreover, the inhibitory activity was not attenuated by the increased amount of infective dose, and a significant reduction of intracellular PRV virions was observed by indirect immunofluorescence. A mode of action study indicated that myricetin could directly inactivate the virus in vitro, leading to inhibition of viral adsorption, penetration and replication in cells. In addition to direct killing effect, myricetin could also activate host antiviral defense through regulation of apoptosis-related gene expressions (Bcl-2, Bcl-xl, Bax), NF-κB and MAPK signaling pathways and cytokine gene expressions (IL-1α, IL-1β, IL-6, c-Jun, STAT1, c-Fos, and c-Myc). In PRV-infected mouse model, myricetin could enhance the survival rate by 40% at 5 days post infection, and viral loads in kidney, liver, lung, spleen, and brain were significantly decreased. The pathological changes caused by PRV infection were improved by myricetin treatment. The gene expressions of inflammatory factors (MCP-1, G-CSF, IL-1α, IL-1β, and IL-6) and apoptotic factors (Bcl-xl, Bcl-2, and Bax) were regulated by myricetin in PRV-infected mice. The present findings suggest that myricetin can effectively inhibit PRV infection and become a candidate for development of new anti-herpesvirus drugs.https://www.frontiersin.org/articles/10.3389/fmicb.2022.985108/fullmyricetindietary flavonoidsherpesviruspseudorabies virusantiviral activity |
| spellingShingle | Huaiyue Hu Zhiqiang Hu Yingying Zhang Hongping Wan Zhongqiong Yin Lixia Li Xiaoxia Liang Xinghong Zhao Lizi Yin Gang Ye Yuan-Feng Zou Huaqiao Tang Renyong Jia Yaqin Chen Hao Zhou Hao Zhou Xu Song Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense Frontiers in Microbiology myricetin dietary flavonoids herpesvirus pseudorabies virus antiviral activity |
| title | Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense |
| title_full | Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense |
| title_fullStr | Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense |
| title_full_unstemmed | Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense |
| title_short | Myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense |
| title_sort | myricetin inhibits pseudorabies virus infection through direct inactivation and activating host antiviral defense |
| topic | myricetin dietary flavonoids herpesvirus pseudorabies virus antiviral activity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.985108/full |
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