Simple generation of site-directed point mutations in the <it>Escherichia coli </it>chromosome using Red<sup>®</sup>/ET<sup>® </sup>Recombination
<p>Abstract</p> <p>Background</p> <p>Introducing point mutations into bacterial chromosomes is important for further progress in studies relying on functional genomics, systems- and synthetic biology, and for metabolic engineering. For many investigations, chromosomal s...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-04-01
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| Series: | Microbial Cell Factories |
| Online Access: | http://www.microbialcellfactories.com/content/7/1/14 |