Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang

Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang

Cpf1 is a promising alternative to Cas9 though indel generation efficiency is target dependent. Here the authors show that the addition of a polyU 3′ overhang can improve the efficiency of low efficiency guide RNAs.

Bibliographic Details
Main Authors: Su Bin Moon, Jeong Mi Lee, Jeong Gu Kang, Nan-Ee Lee, Dae-In Ha, Do Yon Kim, Sun Hee Kim, Kwangsun Yoo, Daesik Kim, Jeong-Heon Ko, Yong-Sam Kim
Format: Article
Language:English
Published: Nature Portfolio 2018-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-06129-w

Internet

https://doi.org/10.1038/s41467-018-06129-w

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