Self-cloning CRISPR/Cpf1 facilitated genome editing in Saccharomyces cerevisiae

Abstract Background Saccharomyces cerevisiae is one of the most important industrial microorganisms. A robust genome editing tool is vital for both fundamental research and applications. To save the time and labor consumed in the procedure of genome editing, a self-cloning CRISPR/Cpf1 system (scCRIS...

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Bibliographic Details
Main Authors: Zhen-Hai Li, Feng-Qing Wang, Dong-Zhi Wei
Format: Article
Language:English
Published: SpringerOpen 2018-07-01
Series:Bioresources and Bioprocessing
Subjects:
Online Access:http://link.springer.com/article/10.1186/s40643-018-0222-8