Self-cloning CRISPR/Cpf1 facilitated genome editing in Saccharomyces cerevisiae
Abstract Background Saccharomyces cerevisiae is one of the most important industrial microorganisms. A robust genome editing tool is vital for both fundamental research and applications. To save the time and labor consumed in the procedure of genome editing, a self-cloning CRISPR/Cpf1 system (scCRIS...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
SpringerOpen
2018-07-01
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Series: | Bioresources and Bioprocessing |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s40643-018-0222-8 |