Effect of Ultrasonic-Assisted Enzymolysis on Protein, Phenolic Content, and Antioxidant Activity of Chlorella sp. Crude Extract
Chlorella sp. microalgae is a potential source of natural antioxidants and other bioactive compounds used in the food and pharmaceutical industries. Ultrasound pretreatments followed by enzymolysis are widely used to improve the functional properties of biological materials. This work aimed to inves...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kementerian Kelautan dan Perikanan
2023-12-01
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| Series: | Squalen |
| Subjects: | |
| Online Access: | http://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/788 |
| _version_ | 1797371258975813632 |
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| author | Setyani Budiari Hamidatun Hamidatun Euis Filailla Nina Artanti Anastasia Fitria Devi Hakiki Melanie Yati Maryati Hani Mulyani Agustine Susilowati Aspiyanto Aspiyanto |
| author_facet | Setyani Budiari Hamidatun Hamidatun Euis Filailla Nina Artanti Anastasia Fitria Devi Hakiki Melanie Yati Maryati Hani Mulyani Agustine Susilowati Aspiyanto Aspiyanto |
| author_sort | Setyani Budiari |
| collection | DOAJ |
| description | Chlorella sp. microalgae is a potential source of natural antioxidants and other bioactive compounds used in the food and pharmaceutical industries. Ultrasound pretreatments followed by enzymolysis are widely used to improve the functional properties of biological materials. This work aimed to investigate the effects of ultrasound pretreatments followed by bromelain hydrolysis on the proteins, phenolic content, and antioxidant activity of Chlorella sp. crude extract. The experimental design for the study involved two factors: ultrasound pretreatment time (0, 5, and 10 min) and bromelain hydrolysis time (0, 2, 4, and 6 hours). The protein was measured using Lowry assay while the total phenolic content was measured using a Folin–Ciocalteu assay. The highest protein content (7.09 ± 0.44 mg/mL) and phenolic concentrations (0.28 ± 0.01 ìg/mL) were obtained at 5 min ultrasound pretreatment and 6 hours enzymolysis. For antioxidant activity, 5 min ultrasound pretreatment followed by 4 hours of enzymolysis resulted in the highest DPPH (1,1-diphenyl-2-picrylhydrazyl radical) inhibitory activity (81.74%). Meanwhile, ultrasound pretreatment for 10 min followed by 6 hours of enzymolysis resulted in the highest ABTS (2,20 azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) inhibitory activity (94.10%). This study showed that variation in ultrasound pretreatments followed by bromelain hydrolysis could be used to improve the protein content, phenolic content, and antioxidant activity of Chlorella sp. crude extract. |
| first_indexed | 2024-03-08T18:17:18Z |
| format | Article |
| id | doaj.art-1aa5a76593734d3f8d47a08131e9c96a |
| institution | Directory Open Access Journal |
| issn | 2089-5690 2406-9272 |
| language | English |
| last_indexed | 2024-03-08T18:17:18Z |
| publishDate | 2023-12-01 |
| publisher | Kementerian Kelautan dan Perikanan |
| record_format | Article |
| series | Squalen |
| spelling | doaj.art-1aa5a76593734d3f8d47a08131e9c96a2023-12-31T09:45:32ZengKementerian Kelautan dan PerikananSqualen2089-56902406-92722023-12-0118310.15578/squalen.788358Effect of Ultrasonic-Assisted Enzymolysis on Protein, Phenolic Content, and Antioxidant Activity of Chlorella sp. Crude ExtractSetyani Budiari0Hamidatun Hamidatun1Euis Filailla2Nina Artanti3Anastasia Fitria Devi4Hakiki Melanie5Yati Maryati6Hani Mulyani7Agustine Susilowati8Aspiyanto Aspiyanto9Research Center for Chemistry, National Research and Innovation Agency, KST BJ Habibie, South Tagerang 15314, IndonesiaSahid university, Jl. Prof. DR. Soepomo No.84, Menteng Dalam Tebet, South Jakarta 12870, IndonesiaResearch Center for Chemistry, National Research and Innovation Agency, KST BJ Habibie, South Tagerang 15314, IndonesiaResearch Center for Chemistry, National Research and Innovation Agency, KST BJ Habibie, South Tagerang 15314, IndonesiaResearch Center for Chemistry, National Research and Innovation Agency, KST BJ Habibie, South Tagerang 15314, IndonesiaResearch Center for Chemistry, National Research and Innovation Agency, KST BJ Habibie, South Tagerang 15314, IndonesiaResearch Center for Chemistry, National Research and Innovation Agency, KST BJ Habibie, South Tagerang 15314, IndonesiaResearch Center for Chemistry, National Research and Innovation Agency, KST BJ Habibie, South Tagerang 15314, IndonesiaResearch Center for Chemistry, National Research and Innovation Agency, KST BJ Habibie, South Tagerang 15314, IndonesiaResearch Center for Chemistry, National Research and Innovation Agency, KST BJ Habibie, South Tagerang 15314, IndonesiaChlorella sp. microalgae is a potential source of natural antioxidants and other bioactive compounds used in the food and pharmaceutical industries. Ultrasound pretreatments followed by enzymolysis are widely used to improve the functional properties of biological materials. This work aimed to investigate the effects of ultrasound pretreatments followed by bromelain hydrolysis on the proteins, phenolic content, and antioxidant activity of Chlorella sp. crude extract. The experimental design for the study involved two factors: ultrasound pretreatment time (0, 5, and 10 min) and bromelain hydrolysis time (0, 2, 4, and 6 hours). The protein was measured using Lowry assay while the total phenolic content was measured using a Folin–Ciocalteu assay. The highest protein content (7.09 ± 0.44 mg/mL) and phenolic concentrations (0.28 ± 0.01 ìg/mL) were obtained at 5 min ultrasound pretreatment and 6 hours enzymolysis. For antioxidant activity, 5 min ultrasound pretreatment followed by 4 hours of enzymolysis resulted in the highest DPPH (1,1-diphenyl-2-picrylhydrazyl radical) inhibitory activity (81.74%). Meanwhile, ultrasound pretreatment for 10 min followed by 6 hours of enzymolysis resulted in the highest ABTS (2,20 azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) inhibitory activity (94.10%). This study showed that variation in ultrasound pretreatments followed by bromelain hydrolysis could be used to improve the protein content, phenolic content, and antioxidant activity of Chlorella sp. crude extract.http://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/788antioxidant activitybromelainchlorella sp.ultrasound |
| spellingShingle | Setyani Budiari Hamidatun Hamidatun Euis Filailla Nina Artanti Anastasia Fitria Devi Hakiki Melanie Yati Maryati Hani Mulyani Agustine Susilowati Aspiyanto Aspiyanto Effect of Ultrasonic-Assisted Enzymolysis on Protein, Phenolic Content, and Antioxidant Activity of Chlorella sp. Crude Extract Squalen antioxidant activity bromelain chlorella sp. ultrasound |
| title | Effect of Ultrasonic-Assisted Enzymolysis on Protein, Phenolic Content, and Antioxidant Activity of Chlorella sp. Crude Extract |
| title_full | Effect of Ultrasonic-Assisted Enzymolysis on Protein, Phenolic Content, and Antioxidant Activity of Chlorella sp. Crude Extract |
| title_fullStr | Effect of Ultrasonic-Assisted Enzymolysis on Protein, Phenolic Content, and Antioxidant Activity of Chlorella sp. Crude Extract |
| title_full_unstemmed | Effect of Ultrasonic-Assisted Enzymolysis on Protein, Phenolic Content, and Antioxidant Activity of Chlorella sp. Crude Extract |
| title_short | Effect of Ultrasonic-Assisted Enzymolysis on Protein, Phenolic Content, and Antioxidant Activity of Chlorella sp. Crude Extract |
| title_sort | effect of ultrasonic assisted enzymolysis on protein phenolic content and antioxidant activity of chlorella sp crude extract |
| topic | antioxidant activity bromelain chlorella sp. ultrasound |
| url | http://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/788 |
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