Antioxidant activity as a response to cadmium pollution in three durum wheat genotypes differing in salt-tolerance
Durum wheat is commonly used in various food industry industries and cultivated worldwide. A serious problem with the species cultivation is its capability to accumulate cadmium (Cd) in the grains. The aim of this study is to investigate whether antioxidant activity may be used as a marker of Cd tol...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2020-09-01
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| Series: | Open Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/chem-2020-0113 |
| _version_ | 1818579689730473984 |
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| author | Pastuszak Jakub Kopeć Przemysław Płażek Agnieszka Gondek Krzysztof Szczerba Anna Hornyák Marta Dubert Franciszek |
| author_facet | Pastuszak Jakub Kopeć Przemysław Płażek Agnieszka Gondek Krzysztof Szczerba Anna Hornyák Marta Dubert Franciszek |
| author_sort | Pastuszak Jakub |
| collection | DOAJ |
| description | Durum wheat is commonly used in various food industry industries and cultivated worldwide. A serious problem with the species cultivation is its capability to accumulate cadmium (Cd) in the grains. The aim of this study is to investigate whether antioxidant activity may be used as a marker of Cd tolerance in durum wheat. The experiment involved three durum wheat genotypes/lines differing in salt tolerance. The plant response to Cd was appraised based on the activity of ascorbate–glutathione (AsA–GSH) cycle enzymes, ascorbate-to-dehydroascorbate ratio, reduced-to-oxidized glutathione ratio (GSH:GSSG), as well as Cd content in the seeds. The highest activity of dehydroascorbate reductase, monodehydroascorbate reductase, and glutathione reductase was noted in control plants of salt-sensitive cultivar “Tamaroi.” In the presence of Cd, activity of these enzymes was considerably reduced. “Tamaroi” plants demonstrated also the highest Cd content in the grain. In conclusion, we identified the cultivar “Tamaroi” as most susceptible to cadmium, and the level of durum wheat sensitivity to the element can be evaluated based on a significant decrease in the activity of AsA–GSH cycle enzymes and GSH:GSSG ratio. |
| first_indexed | 2024-12-16T07:05:42Z |
| format | Article |
| id | doaj.art-1aa6051e5b934df287551a3e9f4fced1 |
| institution | Directory Open Access Journal |
| issn | 2391-5420 |
| language | English |
| last_indexed | 2024-12-16T07:05:42Z |
| publishDate | 2020-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Chemistry |
| spelling | doaj.art-1aa6051e5b934df287551a3e9f4fced12022-12-21T22:40:03ZengDe GruyterOpen Chemistry2391-54202020-09-011811230124110.1515/chem-2020-0113chem-2020-0113Antioxidant activity as a response to cadmium pollution in three durum wheat genotypes differing in salt-tolerancePastuszak Jakub0Kopeć Przemysław1Płażek Agnieszka2Gondek Krzysztof3Szczerba Anna4Hornyák Marta5Dubert Franciszek6Department of Plant Breeding, Physiology and Seed Science, University of Agriculture, ul. Podłużna 3, 30-239 Kraków, PolandDepartment of Cell Biology, The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, PolandDepartment of Plant Breeding, Physiology and Seed Science, University of Agriculture, ul. Podłużna 3, 30-239 Kraków, PolandDepartment of Agriculture and Environmental Chemistry, University of Agriculture, Al. A. Mickiewicza 21, 31-120 Kraków, PolandDepartment of Plant Breeding, Physiology and Seed Science, University of Agriculture, ul. Podłużna 3, 30-239 Kraków, PolandDepartment of Plant Breeding, Physiology and Seed Science, University of Agriculture, ul. Podłużna 3, 30-239 Kraków, PolandDepartment of Cell Biology, The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, ul. Niezapominajek 21, 30-239 Kraków, PolandDurum wheat is commonly used in various food industry industries and cultivated worldwide. A serious problem with the species cultivation is its capability to accumulate cadmium (Cd) in the grains. The aim of this study is to investigate whether antioxidant activity may be used as a marker of Cd tolerance in durum wheat. The experiment involved three durum wheat genotypes/lines differing in salt tolerance. The plant response to Cd was appraised based on the activity of ascorbate–glutathione (AsA–GSH) cycle enzymes, ascorbate-to-dehydroascorbate ratio, reduced-to-oxidized glutathione ratio (GSH:GSSG), as well as Cd content in the seeds. The highest activity of dehydroascorbate reductase, monodehydroascorbate reductase, and glutathione reductase was noted in control plants of salt-sensitive cultivar “Tamaroi.” In the presence of Cd, activity of these enzymes was considerably reduced. “Tamaroi” plants demonstrated also the highest Cd content in the grain. In conclusion, we identified the cultivar “Tamaroi” as most susceptible to cadmium, and the level of durum wheat sensitivity to the element can be evaluated based on a significant decrease in the activity of AsA–GSH cycle enzymes and GSH:GSSG ratio.https://doi.org/10.1515/chem-2020-0113antioxidant enzymesascorbate–glutathione cycleheavy metal stresscadmiumdurum wheat |
| spellingShingle | Pastuszak Jakub Kopeć Przemysław Płażek Agnieszka Gondek Krzysztof Szczerba Anna Hornyák Marta Dubert Franciszek Antioxidant activity as a response to cadmium pollution in three durum wheat genotypes differing in salt-tolerance Open Chemistry antioxidant enzymes ascorbate–glutathione cycle heavy metal stress cadmium durum wheat |
| title | Antioxidant activity as a response to cadmium pollution in three durum wheat genotypes differing in salt-tolerance |
| title_full | Antioxidant activity as a response to cadmium pollution in three durum wheat genotypes differing in salt-tolerance |
| title_fullStr | Antioxidant activity as a response to cadmium pollution in three durum wheat genotypes differing in salt-tolerance |
| title_full_unstemmed | Antioxidant activity as a response to cadmium pollution in three durum wheat genotypes differing in salt-tolerance |
| title_short | Antioxidant activity as a response to cadmium pollution in three durum wheat genotypes differing in salt-tolerance |
| title_sort | antioxidant activity as a response to cadmium pollution in three durum wheat genotypes differing in salt tolerance |
| topic | antioxidant enzymes ascorbate–glutathione cycle heavy metal stress cadmium durum wheat |
| url | https://doi.org/10.1515/chem-2020-0113 |
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