Antigen density quantification of cell-surface immunotherapy targets by flow cytometry: Multi-antigen assay of neuroblastoma bone marrow metastasis

Summary: The central role of target antigen density on chimeric antigen receptor T cell potency highlights the need for accurate measurement of antigen levels on clinical tumor samples. Here, we present a protocol for quantifying antigen density for six cell-surface antigens on neuroblastoma cells m...

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Bibliographic Details
Main Authors: Molly T. Radosevich, Scott J. Bornheimer, Majid Mehrpouryan, Bita Sahaf, Jean S. Oak, Crystal L. Mackall, Sabine Heitzeneder
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723006767