Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming

Summary: Understanding how changes in gut microbiota in early life impact immune programming can be difficult to study due to variations in the assembly of the microbiota. In this protocol, we describe how to colonize gnotobiotic/germ-free mice in early life with different microbiota community types...

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Bibliographic Details
Main Authors: Miriam A. Lynn, Feargal J. Ryan, Yee Chern Tee, David J. Lynn
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007948