Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming
Summary: Understanding how changes in gut microbiota in early life impact immune programming can be difficult to study due to variations in the assembly of the microbiota. In this protocol, we describe how to colonize gnotobiotic/germ-free mice in early life with different microbiota community types...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722007948 |
| _version_ | 1797980478718869504 |
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| author | Miriam A. Lynn Feargal J. Ryan Yee Chern Tee David J. Lynn |
| author_facet | Miriam A. Lynn Feargal J. Ryan Yee Chern Tee David J. Lynn |
| author_sort | Miriam A. Lynn |
| collection | DOAJ |
| description | Summary: Understanding how changes in gut microbiota in early life impact immune programming can be difficult to study due to variations in the assembly of the microbiota. In this protocol, we describe how to colonize gnotobiotic/germ-free mice in early life with different microbiota community types (e.g., PAMI and PAMII). We detail several assays to determine whether differential colonization alters immune programming in early life. We also describe how to propagate mouse fecal microbiota transplant material if the donor fecal sample is limited.For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T05:55:03Z |
| format | Article |
| id | doaj.art-1aef87eda5e0461f888b56ebca24484d |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T05:55:03Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-1aef87eda5e0461f888b56ebca24484d2022-12-22T04:41:54ZengElsevierSTAR Protocols2666-16672022-12-0134101914Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programmingMiriam A. Lynn0Feargal J. Ryan1Yee Chern Tee2David J. Lynn3Precision Medicine Theme, South Australian Health and Medical Research Institute, Adelaide, SA 5001, Australia; Flinders Health and Medical Research Institute, Flinders University, Bedford Park, SA 5042, Australia; Corresponding authorPrecision Medicine Theme, South Australian Health and Medical Research Institute, Adelaide, SA 5001, Australia; Flinders Health and Medical Research Institute, Flinders University, Bedford Park, SA 5042, AustraliaPrecision Medicine Theme, South Australian Health and Medical Research Institute, Adelaide, SA 5001, Australia; Flinders Health and Medical Research Institute, Flinders University, Bedford Park, SA 5042, AustraliaPrecision Medicine Theme, South Australian Health and Medical Research Institute, Adelaide, SA 5001, Australia; Flinders Health and Medical Research Institute, Flinders University, Bedford Park, SA 5042, Australia; Corresponding authorSummary: Understanding how changes in gut microbiota in early life impact immune programming can be difficult to study due to variations in the assembly of the microbiota. In this protocol, we describe how to colonize gnotobiotic/germ-free mice in early life with different microbiota community types (e.g., PAMI and PAMII). We detail several assays to determine whether differential colonization alters immune programming in early life. We also describe how to propagate mouse fecal microbiota transplant material if the donor fecal sample is limited.For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722007948ImmunologyMicrobiologyModel OrganismsMolecular Biology |
| spellingShingle | Miriam A. Lynn Feargal J. Ryan Yee Chern Tee David J. Lynn Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming STAR Protocols Immunology Microbiology Model Organisms Molecular Biology |
| title | Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming |
| title_full | Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming |
| title_fullStr | Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming |
| title_full_unstemmed | Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming |
| title_short | Protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming |
| title_sort | protocol to colonize gnotobiotic mice in early life and assess the impact on early life immune programming |
| topic | Immunology Microbiology Model Organisms Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722007948 |
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