Examining MLKL phosphorylation to detect necroptosis in murine mammary tumors

Summary: Necroptosis occurs predominantly in the center of late-stage tumors and necroptotic cells are dispersed and difficult to be detected by Western blotting of key markers without enrichment by microdissection. To overcome these obstacles, this protocol provides a detailed immunohistochemistry-...

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Bibliographic Details
Main Authors: Jin Young Baik, Peixing Wan, Zheng-Gang Liu
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722003379