Mutagenesis and Gene Cloning in Schizosaccharomyces pombe Using Nonhomologous Plasmid Integration and Rescue
Genes are commonly cloned in yeasts and bacteria by plasmid complementation, where the introduction of the gene of interest into a host strain carrying a recessive mutation in that gene suppresses the host’s mutant phenotype. However, a lack of low copy cloning vectors in the fission yeast Schizosac...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2000-03-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/00283rr02 |