Identifying parameters to improve the reproducibility of transient gene expression in High Five cells.

Virus-free, transient gene expression (TGE) in High Five cells was recently presented as an efficient protein production method. However, published TGE protocols have not been standardized to a general protocol. Therefore, reproducibility and implementation of the method in other labs remains diffic...

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Bibliographic Details
Main Authors: Maren Bleckmann, Margitta Schürig, Michelle Endres, Anke Samuels, Daniela Gebauer, Nadine Konisch, Joop van den Heuvel
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0217878