Automated segmentation and quantitative analysis of organelle morphology, localization and content using CellProfiler.

One of the most used and versatile methods to study number, dimensions, content and localization of secretory organelles is confocal microscopy analysis. However, considerable heterogeneity exists in the number, size and shape of secretory organelles that can be present in the cell. One thus needs t...

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Bibliographic Details
Main Authors: Sebastiaan N J Laan, Richard J Dirven, Petra E Bürgisser, Jeroen Eikenboom, Ruben Bierings, SYMPHONY consortium
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2023-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0278009