Protocol for electrotaxis of large epithelial cell sheets

Protocol for electrotaxis of large epithelial cell sheets

Summary: Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size a...

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Bibliographic Details
Main Authors: Yan Zhang, Rachel M. Lee, Zijie Zhu, Yaohui Sun, Kan Zhu, Zhengping Xu, Francis Lin, Tingrui Pan, Wolfgang Losert, Min Zhao
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Biophysics
Biotechnology and bioengineering
Cell Biology
Cell-based Assays
Microscopy
Tissue Engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002551

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http://www.sciencedirect.com/science/article/pii/S2666166723002551

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