Protocol for electrotaxis of large epithelial cell sheets
Summary: Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size a...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723002551 |
| _version_ | 1797831663785345024 |
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| author | Yan Zhang Rachel M. Lee Zijie Zhu Yaohui Sun Kan Zhu Zhengping Xu Francis Lin Tingrui Pan Wolfgang Losert Min Zhao |
| author_facet | Yan Zhang Rachel M. Lee Zijie Zhu Yaohui Sun Kan Zhu Zhengping Xu Francis Lin Tingrui Pan Wolfgang Losert Min Zhao |
| author_sort | Yan Zhang |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size and shape of human keratinocyte cell sheets. We detail cell tracking, cell sheet contour assay, and particle image velocimetry to reveal the spatial and temporal motility dynamics of cell sheets. This approach is applicable to other collective cell migration studies.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-09T13:56:30Z |
| format | Article |
| id | doaj.art-1b3e0f7478c9470594db3abafac19b06 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-09T13:56:30Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-1b3e0f7478c9470594db3abafac19b062023-05-08T04:10:02ZengElsevierSTAR Protocols2666-16672023-06-0142102288Protocol for electrotaxis of large epithelial cell sheetsYan Zhang0Rachel M. Lee1Zijie Zhu2Yaohui Sun3Kan Zhu4Zhengping Xu5Francis Lin6Tingrui Pan7Wolfgang Losert8Min Zhao9Department of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USA; School of Public Health, Hangzhou Normal University, Hangzhou 311121, China; Institute of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China; Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, California, Davis, CA 95616, USA; Corresponding authorInstitute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USAMicro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, California, Davis, CA 95616, USADepartment of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USADepartment of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USAInstitute of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou 310058, ChinaDepartment of Physics and Astronomy, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaMicro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, California, Davis, CA 95616, USA; Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Shenzhen 518055, China; Shenzhen Engineering Laboratory of Single-molecule Detection and Instrument Development, Shenzhen, Guangdong 518055, China; Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou 215123, ChinaInstitute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA; Department of Physics, University of Maryland, College Park, MD 20742, USADepartment of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USA; Department of Dermatology, University of California, California, Davis, CA 95616, USA; Corresponding authorSummary: Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size and shape of human keratinocyte cell sheets. We detail cell tracking, cell sheet contour assay, and particle image velocimetry to reveal the spatial and temporal motility dynamics of cell sheets. This approach is applicable to other collective cell migration studies.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723002551BiophysicsBiotechnology and bioengineeringCell BiologyCell-based AssaysMicroscopyTissue Engineering |
| spellingShingle | Yan Zhang Rachel M. Lee Zijie Zhu Yaohui Sun Kan Zhu Zhengping Xu Francis Lin Tingrui Pan Wolfgang Losert Min Zhao Protocol for electrotaxis of large epithelial cell sheets STAR Protocols Biophysics Biotechnology and bioengineering Cell Biology Cell-based Assays Microscopy Tissue Engineering |
| title | Protocol for electrotaxis of large epithelial cell sheets |
| title_full | Protocol for electrotaxis of large epithelial cell sheets |
| title_fullStr | Protocol for electrotaxis of large epithelial cell sheets |
| title_full_unstemmed | Protocol for electrotaxis of large epithelial cell sheets |
| title_short | Protocol for electrotaxis of large epithelial cell sheets |
| title_sort | protocol for electrotaxis of large epithelial cell sheets |
| topic | Biophysics Biotechnology and bioengineering Cell Biology Cell-based Assays Microscopy Tissue Engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723002551 |
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