Creation of targeted genomic deletions using TALEN or CRISPR/Cas nuclease pairs in one‐cell mouse embryos

Creation of targeted genomic deletions using TALEN or CRISPR/Cas nuclease pairs in one‐cell mouse embryos

The use of TALEN and CRISPR/CAS nucleases is becoming increasingly popular as a means to edit single target sites in one‐cell mouse embryos. Nevertheless, an area that has received less attention concerns the engineering of structural genome variants and the necessary religation of two distant doubl...

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Bibliographic Details
Main Authors: Christina Brandl, Oskar Ortiz, Bernhard Röttig, Benedikt Wefers, Wolfgang Wurst, Ralf Kühn
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:FEBS Open Bio
Subjects:
TALENs
Disease model
One-cell embryo
Mouse mutant
CRISPR
Cas9
Online Access:https://doi.org/10.1016/j.fob.2014.11.009

Internet

https://doi.org/10.1016/j.fob.2014.11.009

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