Chromatin structure and context-dependent sequence features control prime editing efficiency

Chromatin structure and context-dependent sequence features control prime editing efficiency

Prime editing (PE) is a highly versatile CRISPR–Cas9 genome editing technique. The current constructs, however, have variable efficiency and may require laborious experimental optimization. This study presents statistical models for learning the salient epigenomic and sequence features of target sit...

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Bibliographic Details
Main Authors: Somang Kim, Jimmy B. Yuan, Wendy S. Woods, Destry A. Newton, Pablo Perez-Pinera, Jun S. Song
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-06-01
Series:Frontiers in Genetics
Subjects:
prime editing
CRISPR–Cas9
heterochromatin
nucleosome positioning
DNA-RNA hybridization
nucleotide preference
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2023.1222112/full

Internet

https://www.frontiersin.org/articles/10.3389/fgene.2023.1222112/full

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