Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers

Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers

ABSTRACT Selectable markers are indispensable for genetic engineering, yet their number and variety are limited. Most selection procedures for prototrophic cells rely on the introduction of antibiotic resistance genes. New minimally invasive tools are needed to facilitate sophisticated genetic manip...

Full description

Bibliographic Details
Main Authors: Lukas Birštonas, Alex Dallemulle, Manuel S. López-Berges, Ilse D. Jacobsen, Martin Offterdinger, Beate Abt, Maria Straßburger, Ingo Bauer, Oliver Schmidt, Bettina Sarg, Herbert Lindner, Hubertus Haas, Fabio Gsaller
Format: Article
Language:English
Published: American Society for Microbiology 2020-04-01
Series:mBio
Subjects:
endogenous selectable markers
genetic engineering
pyrimidine salvage pathway
targeted genomic integrations
Online Access:https://journals.asm.org/doi/10.1128/mBio.00230-20

Internet

https://journals.asm.org/doi/10.1128/mBio.00230-20

Similar Items