Scaling up production of recombinant human basic fibroblast growth factor in an Escherichia coli BL21(DE3) plysS strain and evaluation of its pro-wound healing efficacy

Scaling up production of recombinant human basic fibroblast growth factor in an Escherichia coli BL21(DE3) plysS strain and evaluation of its pro-wound healing efficacy

Introduction: Human basic fibroblast growth factor (hbFGF) is a highly valuable multifunctional protein that plays a crucial role in various biological processes. In this study, we aim to accomplish the scaling-up production of mature hbFGF (146aa) by implementing a high cell-density fermentation an...

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Bibliographic Details
Main Authors: Le Li, Bingjie Yu, Yingji Lai, Siyuan Shen, Yawei Yan, Guojun Dong, Xiangyun Gao, Yanrong Cao, Caojie Ge, Liqin Zhu, Huan Liu, Shanhui Tao, Zhiang Yao, Shijun Li, Xiaojie Wang, Qi Hui
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-02-01
Series:Frontiers in Pharmacology
Subjects:
hbFGF
Escherichia coli BL21(DE3) plysS
optimized production
500-L fermentation
purification
wound healing
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2023.1279516/full

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https://www.frontiersin.org/articles/10.3389/fphar.2023.1279516/full

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