Hydroxyapatite chromatography of phage-display virions
Hydroxyapatite column chromatography can be used to purify filamentous bacteriophage—the phage most commonly used for phage display. Virions that have been partially purified from culture supernatant by two cycles of precipitation in 2% polyethylene glycol are adsorbed onto the matrix at a density o...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2005-12-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112032 |