GSK3 inhibitor-BIO regulates proliferation of immortalized pancreatic mesenchymal stem cells (iPMSCs).
BACKGROUND: The small molecule 6-bromoindirubin-30-oxime (BIO), a glycogen synthase kinase 3 (GSK3) inhibitor, is a pharmacological agent known to maintain self-renewal in human and mouse embryonic stem cells (ESCs). However, the precise role of GSK3 in immortalized pancreatic mesenchymal stem cells...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3285662?pdf=render |
| _version_ | 1818063343286484992 |
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| author | Hui Cao Yuankui Chu Xiao Lv Pubin Qiu Chao Liu Huiru Zhang Dan Li Sha Peng Zhongying Dou Jinlian Hua |
| author_facet | Hui Cao Yuankui Chu Xiao Lv Pubin Qiu Chao Liu Huiru Zhang Dan Li Sha Peng Zhongying Dou Jinlian Hua |
| author_sort | Hui Cao |
| collection | DOAJ |
| description | BACKGROUND: The small molecule 6-bromoindirubin-30-oxime (BIO), a glycogen synthase kinase 3 (GSK3) inhibitor, is a pharmacological agent known to maintain self-renewal in human and mouse embryonic stem cells (ESCs). However, the precise role of GSK3 in immortalized pancreatic mesenchymal stem cells (iPMSCs) growth and survival is not completely understood at present. RESULTS: To determine whether this molecule is involved in controlling the proliferation of iPMSCs, we examined the effect of BIO on iPMSCs. We found that the inactivation of GSK3 by BIO can robustly stimulate iPMSCs proliferation and mass formation as shown by QRT-PCR, western blotting, 5-Bromo-2-deoxyuridine (BrdU) immunostaining assay and tunel assay. However, we did not find the related roles of BIO on β cell differentiation by immunostaining, QRT-PCR assay, glucose-stimulated insulin release and C-peptide content analysis. CONCLUSIONS: These results suggest that BIO plays a key role in the regulation of cell mass proliferation and maintenance of the undifferentiated state of iPMSCs. |
| first_indexed | 2024-12-10T14:18:36Z |
| format | Article |
| id | doaj.art-1ba97270098b4d0b9de708345ad9d6ec |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T14:18:36Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1ba97270098b4d0b9de708345ad9d6ec2022-12-22T01:45:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3150210.1371/journal.pone.0031502GSK3 inhibitor-BIO regulates proliferation of immortalized pancreatic mesenchymal stem cells (iPMSCs).Hui CaoYuankui ChuXiao LvPubin QiuChao LiuHuiru ZhangDan LiSha PengZhongying DouJinlian HuaBACKGROUND: The small molecule 6-bromoindirubin-30-oxime (BIO), a glycogen synthase kinase 3 (GSK3) inhibitor, is a pharmacological agent known to maintain self-renewal in human and mouse embryonic stem cells (ESCs). However, the precise role of GSK3 in immortalized pancreatic mesenchymal stem cells (iPMSCs) growth and survival is not completely understood at present. RESULTS: To determine whether this molecule is involved in controlling the proliferation of iPMSCs, we examined the effect of BIO on iPMSCs. We found that the inactivation of GSK3 by BIO can robustly stimulate iPMSCs proliferation and mass formation as shown by QRT-PCR, western blotting, 5-Bromo-2-deoxyuridine (BrdU) immunostaining assay and tunel assay. However, we did not find the related roles of BIO on β cell differentiation by immunostaining, QRT-PCR assay, glucose-stimulated insulin release and C-peptide content analysis. CONCLUSIONS: These results suggest that BIO plays a key role in the regulation of cell mass proliferation and maintenance of the undifferentiated state of iPMSCs.http://europepmc.org/articles/PMC3285662?pdf=render |
| spellingShingle | Hui Cao Yuankui Chu Xiao Lv Pubin Qiu Chao Liu Huiru Zhang Dan Li Sha Peng Zhongying Dou Jinlian Hua GSK3 inhibitor-BIO regulates proliferation of immortalized pancreatic mesenchymal stem cells (iPMSCs). PLoS ONE |
| title | GSK3 inhibitor-BIO regulates proliferation of immortalized pancreatic mesenchymal stem cells (iPMSCs). |
| title_full | GSK3 inhibitor-BIO regulates proliferation of immortalized pancreatic mesenchymal stem cells (iPMSCs). |
| title_fullStr | GSK3 inhibitor-BIO regulates proliferation of immortalized pancreatic mesenchymal stem cells (iPMSCs). |
| title_full_unstemmed | GSK3 inhibitor-BIO regulates proliferation of immortalized pancreatic mesenchymal stem cells (iPMSCs). |
| title_short | GSK3 inhibitor-BIO regulates proliferation of immortalized pancreatic mesenchymal stem cells (iPMSCs). |
| title_sort | gsk3 inhibitor bio regulates proliferation of immortalized pancreatic mesenchymal stem cells ipmscs |
| url | http://europepmc.org/articles/PMC3285662?pdf=render |
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